EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes
| Autor: | Liliana B. Areces, Giulio Draetta, Maddalena Donzelli, Monica Mancino, Massimo Squatrito |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cytoplasm
Cancer Research Macromolecular Substances Nucleolus Recombinant Fusion Proteins Amino Acid Motifs Molecular Sequence Data Biology Transfection Ribosome Ribosome assembly Mice RNA Precursors Genetics Animals Humans Amino Acid Sequence RRNA processing Molecular Biology Adaptor Proteins Signal Transducing Sequence Deletion Ribonucleoprotein Sequence Homology Amino Acid Cell growth RNA-Binding Proteins 3T3 Cells Fibroblasts Ribosomal RNA Molecular biology Protein Structure Tertiary Cell biology Amino Acid Substitution Ribonucleoproteins Organ Specificity RNA Ribosomal Carrier Proteins Ribosomes Sequence Alignment Cell Division Cell Nucleolus HeLa Cells |
| Zdroj: | Oncogene. 23:4454-4465 |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1207579 |
| Popis: | EBP1 was identified as a protein that interacts with the ErbB-3 receptor and possibly contributes to transducing growth regulatory signals. The existence of EBP1 homologs across species from simple eukaryotes to humans and its wide tissue expression pattern suggest that EBP1 acts as a general signaling molecule. We provide evidence that EBP1 is localized to the cytoplasm and to the nucleolus, and that its nucleolar localization requires amino-acid sequences present at both the amino- and carboxy-terminus of the molecule. We also show that EBP1 overexpression inhibits proliferation of human fibroblasts, and that this effect is linked to its nucleolar localization. Using mass spectrometry we demonstrate that EBP1 is part of ribonucleoprotein complexes and associates with different rRNA species. It is becoming clear that cell growth and proliferation are actively coordinated with rRNA processing and ribosome assembly. Our findings indicate that EBP1 is a nucleolar growth-regulating protein, and we propose that it could represent a new link between ribosome biosynthesis and cell proliferation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|