Soft Polydimethylsiloxane-Supported Lipid Bilayers for Studying T Cell Interactions
| Autor: | Edward Jenkins, Simon J. Davis, David Klenerman, Jane Humphrey, Ana Filipa L.O.M. Santos, Ivan B Dimov, James McColl, Alexander K. Winkel, Anna Lippert, Kevin Y. Chen, Kristian Franze |
|---|---|
| Přispěvatelé: | Lippert, Anna [0000-0003-0463-6535], Humphrey, Jane [0000-0002-6825-1426], Franze, Kristian [0000-0002-8425-7297], Klenerman, David [0000-0001-7116-6954], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Polydimethylsiloxane Chemistry T-Lymphocytes T cell Lipid Bilayers Biophysics Substrate (chemistry) Articles Cell Communication Mechanical resistance 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure Elastic Modulus medicine Mechanosensitive channels Dimethylpolysiloxanes Receptor Lipid bilayer 030217 neurology & neurosurgery 030304 developmental biology Calcium signaling |
| Zdroj: | Biophysical Journal |
| ISSN: | 0006-3495 |
| Popis: | Much of what we know about the early stages of T cell activation has been obtained from studies of T cells interacting with glass-supported lipid bilayers that favor imaging but are orders of magnitude stiffer than typical cells. We developed a method for attaching lipid bilayers to polydimethylsiloxane polymer supports, producing “soft bilayers” with physiological levels of mechanical resistance (Young’s modulus of 4 kPa). Comparisons of T cell behavior on soft and glass-supported bilayers revealed that whereas late stages of T cell activation are thought to be substrate-stiffness dependent, early calcium signaling was unaffected by substrate rigidity, implying that early steps in T cell receptor triggering are not mechanosensitive. The exclusion of large receptor-type phosphatases was observed on the soft bilayers, however, even though it is yet to be demonstrated at authentic cell-cell contacts. This work sets the stage for an imaging-based exploration of receptor signaling under conditions closely mimicking physiological cell-cell contact. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|