A rational approach for cancer stem-like cell isolation and characterization using CD44 and prominin-1(CD133) as selection markers
| Autor: | Shih-Chung Hsu, Ming-Ching Kao, Chien-Chih Ou, Yi-Jen Lee, Hsiu-Hsueh Tseng, Jah-Yao Liu, Chang-Cheng Wu, Jhy-Wei Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Time Factors endocrine system diseases Cell Separation Mice SCID SKOV3.PX1_133+44+ Ovarian tumor 0302 clinical medicine Obstetrics and gynaecology Cell Movement Mice Inbred NOD Ascites Tumor Cells Cultured Ascitic Fluid AC133 Antigen Cell Self Renewal Neoplasm Metastasis Ovarian Neoplasms biology Cell Differentiation Flow Cytometry female genital diseases and pregnancy complications Tumor Burden intraperitoneal enrichment Hyaluronan Receptors Phenotype 030220 oncology & carcinogenesis Neoplastic Stem Cells Female medicine.symptom CD44 and CD133 Research Paper medicine.medical_specialty OVCAR3.PX1_133+44+ Mice Nude Antineoplastic Agents 03 medical and health sciences Cancer stem cell Internal medicine Cell Line Tumor medicine Biomarkers Tumor Animals Humans Cell Lineage Cell Proliferation Dose-Response Relationship Drug business.industry cancer stem-like cells (CSLC) CD44 Cancer medicine.disease Cystadenocarcinoma Serous Transplantation 030104 developmental biology Drug Resistance Neoplasm Immunology biology.protein Ovarian cancer business |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Yi-Jen Lee 1 , Chang-Cheng Wu 2 , Jhy-Wei Li 3, 4 , Chien-Chih Ou 5 , Shih-Chung Hsu 6 , Hsiu-Hsueh Tseng 7 , Ming-Ching Kao 8, 9 , Jah-Yao Liu 10, 11 1 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan 2 Chief of Obstetrics and Gynecology, Tri-Service General Hospital Penghu Branch, Penghu, Taiwan 3 Chief of Pathology, Da-Chien General Hospital, Miaoli, Taiwan 4 Department of Rehabilitation science, Jente Junior College of Medicine, Nursing and Management, Miaoli, Taiwan 5 Obstetrics and Gynecology, Tri-Service General Hospital, Taipei, Taiwan 6 Medical Care and Management, Kang-Ning Junior College, Taipei, Taiwan 7 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan 8 Department of Biological Science and Technology, China Medical University, Taichung, Taiwan 9 Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan 10 Department of Obstetrics and Gynecology, National Defense Medical Center, Taipei, Taiwan 11 Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan Correspondence to: Jah-Yao Liu, email: 8339liu@gmail.com Keywords: cancer stem-like cells (CSLC), intraperitoneal enrichment, CD44 and CD133, SKOV3.PX1_133 + 44 + , OVCAR3.PX1_133 + 44 + Received: April 13, 2015 Accepted: September 12, 2016 Published: September 17, 2016 ABSTRACT The availability of adequate cancer stem cells or cancer stem-like cell (CSC) is important in cancer study. From ovarian cancer cell lines, SKOV3 and OVCAR3, we induced peritoneal ascites tumors in immunodeficient mice. Among the cells (SKOV3.PX1 and OVCAR3.PX1) from those tumors, we sorted both CD44 and CD133 positive cells (SKOV3.PX1_133 + 44 + , OVCAR3.PX1_133 + 44 + ), which manifest the characteristics of self-renewal, multi-lineage differentiation, chemoresistance and tumorigenicity, those of cancer stem-like cells (CSLC). Intraperitoneal transplantation of these CD44 and CD133 positive cells resulted in poorer survival in the engrafted animals. Clinically, increased CD133 expression was found in moderately and poorly differentiated (grade II and III) ovarian serous cystadenocarcinomas. The ascites tumor cells from human ovarian cancers demonstrated more CD133 and CD44 expressions than those from primary ovarian or metastatic tumors and confer tumorigenicity in immunodeficient mice. Compared to their parental cells, the SKOV3.PX1_133 + 44 + and OVCAR3.PX1_133 + 44 + cells uniquely expressed 5 CD markers (CD97, CD104, CD107a, CD121a, and CD125). Among these markers, CD97, CD104, CD107a, and CD121a are significantly more expressed in the CD133+ and CD44+ double positive cells of human ovarian ascites tumor cells (Ascites_133 + 44 + ) than those from primary ovarian or metastatic tumors. The cancer stem-like cells were enriched from 3% to more than 70% after this manipulation. This intraperitoneal enrichment of cancer stem-like cells, from ovarian cancer cell lines or primary ovarian tumor, potentially provides an adequate amount of ovarian cancer stem-like cells for the ovarian cancer study and possibly benefits cancer therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|