Chronic arsenic exposure lowered sperm motility via impairing ultra-microstructure and key proteins expressions of sperm acrosome and flagellum formation during spermiogenesis in male mice
Autor: | Jianhai Zhang, Ram Kumar Manthari, Jie Yang, Xiang Li, Chenkai Cheng, Yin Xing, Yuxiang Yu, Yanjia Tan, Xiaolin Tian, Chen Liang, Jundong Wang, Weixiang Fu, Wei Yang, Yongli Han |
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Rok vydání: | 2020 |
Předmět: |
Male
Environmental Engineering 010504 meteorology & atmospheric sciences Spermiogenesis A Kinase Anchor Proteins 010501 environmental sciences Flagellum Biology 01 natural sciences Arsenic Male infertility Andrology Mice medicine Animals Environmental Chemistry Spermatogenesis Acrosome Waste Management and Disposal Sperm motility 0105 earth and related environmental sciences Sperm flagellum Membrane Proteins medicine.disease Spermatozoa Pollution Sperm Flagella Sperm Motility Reproductive toxicity |
Zdroj: | Science of The Total Environment. 734:139233 |
ISSN: | 0048-9697 |
Popis: | Arsenic (As) poisoning and its potential reproductive functional lesions are a global environmental concern. Recent studies shown that spermiogenesis tends to be a major target process in arsenic-induced male infertility, however, the underlying mechanisms are not fully illuminated. In the present study, 32 fertility related indices including sperm motility, dynamic acrosome formation and sperm flagellum during spermiogenesis in testes were evaluated in adult male mice treated with 0, 0.2, 2, and 20 ppm As2O3 via drinking water for 180 consecutive days. The results showed that out of 32 indices, 11, 25, and 29 indicators were changed statistically by 0.2-, 2-, and 20- ppm As2O3 treatment compared to the controls (0 ppm As2O3), respectively, which reveals a significant dose-dependent relationship. For details, sperm motilities were significantly decreased by 18.85%, 32.47% and 29.53% in three As2O3 treatment groups compared to the control group. Meanwhile, the ultra-structures of acrosome formation and sperm flagellum in testes have been altered by chronic arsenic exposure. Furthermore, arsenic decreased the mRNA expressions of 11 out of 13 genes associated with acrosome biosynthesis and 11 out of 12 genes related to flagellum formation in testes, particularly, down-regulated DPY19L2, AKAP3, AKAP4, CFAP44 and SPAG16 were further confirmed at the protein levels by western blotting. Taken together, chronic arsenic exposure declines male fertility by disorganizing dynamic acrosome and flagellum formation in testes. Especially, DPY19L2, AKAP3, AKAP4, CFAP44, and SPAG16 maybe the potential targets in this process. These results may offer not only a new insight to the mechanism of arsenic-induced male reproductive toxicity, but also provide a clue for the diagnosis and therapy of arseniasis. |
Databáze: | OpenAIRE |
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