l-proline supplementation improves nitric oxide bioavailability and counteracts the blood pressure rise induced by angiotensin II in rats
| Autor: | Maria de Lourdes Bastos, Joana Afonso, António Albino-Teixeira, Luísa Teixeira-Santos, Jorge Carvalho, Joana Barreiros Leal, Teresa Sousa, Sónia Fraga, Dora Pinho |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cancer Research medicine.medical_specialty Antioxidant Proline Physiology medicine.medical_treatment Urinary system Clinical Biochemistry Biological Availability Blood Pressure 030204 cardiovascular system & hematology Nitric Oxide Biochemistry Doenças Cardio e Cérebro-vasculares Nitric oxide Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Saline Angiotensin II Rats 3. Good health Bioavailability 030104 developmental biology Endocrinology Blood pressure chemistry Dietary Supplements Hypertension cardiovascular system Asymmetric dimethylarginine hormones hormone substitutes and hormone antagonists circulatory and respiratory physiology |
| Zdroj: | Nitric Oxide. 82:1-11 |
| ISSN: | 1089-8603 |
| Popis: | We evaluated whether l-proline (Pro) supplementation improves redox status and nitric oxide (NO) bioavailability and prevents or delays angiotensin II (AngII)-induced hypertension. Male Sprague-Dawley rats were distributed to four experimental groups: Pro + AngII (Pro-Ang), Pro + Saline (Pro-Sal), Vehicle + AngII (Veh-Ang) and Veh + Saline (Veh-Sal). Pro solution (2 g.kg-1·day-1) or water (vehicle) were orally administered, from day 0 to day 21. AngII (200 ng.kg-1.min-1) or saline were infused (s.c.) from day 7 to day 21. Systolic blood pressure (SBP) was measured by the tail-cuff method. From day 20-21, animals were kept on metabolic cages for 24h-urine collection. On day 21, urine and blood were collected for further quantification of redox status biomarkers, NO-related markers (urinary nitrates and nitrites, U-NOx; plasma asymmetric dimethylarginine, P-ADMA), metabolic and renal parameters. Pro prevented the AngII-induced SBP rise [mean (95% CI), Day 19: Pro-AngII, 137 (131; 143) vs. Veh-AngII, 157 (151; 163) mm Hg, P |
| Databáze: | OpenAIRE |
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