Friedelane, isolated from Pouzolzia indica Gaud. exhibits toxic effect against melanoma
| Autor: | Indrajit Sil Sarma, Kuntal Manna, Biswanath Dinda, Soham Sarkar, Ashish Kumar Singha, Debasish Maiti, Chaitali Sarkar |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Acridine orange Biomedical Engineering Bioengineering Cell Biology Molecular biology Staining 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Cell culture Apoptosis 030220 oncology & carcinogenesis Original Article Trypan blue MTT assay Viability assay Cytotoxicity Biotechnology |
| Zdroj: | Cytotechnology. 70:1111-1120 |
| ISSN: | 1573-0778 0920-9069 |
| Popis: | Melanoma is a predominant cause of skin cancer-related deaths. It was reported that, the methanolic extract of Pouzolzia Indica (P. indica) on chromatography gave five compounds (1-hentriacontanyl palmitate, myricyl alcohol, 6,7-dimethoxycoumarin, trichadonic acid and friedelane), which inhibited the acute promyelocytic leukemia cell lines, NB4, and HT93A. Friedelane was extracted as active compound from methanolic extract of P. indica. In this study, friedelane was tested on murine metastatic B16F10 and B16BL6 melanoma cell lines. To achieve the target, the cell viability using trypan blue exclusion, acridine orange/EtBr staining and cell cytotoxicity were tested using MTT assay. Caspase-3, caspase-9, Cyt-c, BAD and Bax protein were assayed to evidence the apoptosis induction. The compound friedelane shows potent cytotoxic effect against metastatic melanoma mouse cell lines in 10 µg/ml concentration. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10616-017-0174-4) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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