Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1
| Autor: | Jeremie Ngezahayo, Sofia Oliveira Ribeiro, Caroline Stevigny, Laurent Pottier, Florence Souard, Léonard Hari, Pierre Duez, Mondher El Jaziri, Tsiry Rasamiravaka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Virulence Factors 030106 microbiology Virulence Biology medicine.disease_cause anti-virulence Catalysis Article biofilm Microbiology Inorganic Chemistry Terpene lcsh:Chemistry 03 medical and health sciences medicine Humans Platostoma rotundifolium Lamiaceae Pseudomonas aeruginosa quorum sensing Pseudomonas Infections Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Regulator gene Terpenes Organic Chemistry Biofilm Quorum Sensing General Medicine Gene Expression Regulation Bacterial Sciences bio-médicales et agricoles Terpenoid Computer Science Applications Anti-Bacterial Agents Quorum sensing 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Biofilms Bacterial Model Sciences exactes et naturelles |
| Zdroj: | International journal of molecular sciences, 18 (6 International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 18; Issue 6; Pages: 1270 International Journal of Molecular Sciences, Vol 18, Iss 6, p 1270 (2017) |
| Popis: | Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability. Bioguided fractionation of the ethyl acetate extract led to the isolation of major anti-virulence compounds that were identified from nuclear magnetic resonance and high-resolution molecular spectroscopy spectra as cassipourol, β-sitosterol and α-amyrin. Globally, cassipourol and β-sitosterol inhibit quorum sensing-regulated and -regulatory genes expression in las and rhl systems without affecting the global regulators gacA and vfr, whereas α-amyrin had no effect on the expression of these genes. These terpenoids disrupt the formation of biofilms at concentrations down to 12.5, 50 and 50 µM for cassipourol, β-sitosterol and α-amyrin, respectively. Moreover, these terpenoids reduce the production of total exopolysaccharides and promote flagella-dependent motilities (swimming and swarming). The isolated terpenoids exert a wide range of inhibition processes, suggesting a complex mechanism of action targeting P. aeruginosa virulence mechanisms which support the wide anti-infectious use of this plant species in traditional Burundian medicine. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|