Effects of let-7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression
Autor: | Wei Zhang, Dongmei Lei, Tianjiao Chu, Zhenying Ban, Wentao Guo, Qingru Zeng, Chi Liu, Xianxu Zeng, Jing Cao |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Cancer Research medicine.diagnostic_test Oncogene Chemistry Cell Transfection Articles Cell cycle Molecular biology Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology Apoptosis 030220 oncology & carcinogenesis microRNA Gene expression medicine |
Zdroj: | Oncology letters. 16(5) |
ISSN: | 1792-1074 |
Popis: | MicroRNAs serve a role in the development of ovarian cancer (OC). The present study investigated whether let-7c is able to regulate the proliferation of OC cells by targeting cell division cycle 25A (CDC25a). The reverse transcription-quantitative polymerase chain reaction was performed to detect the expression of let-7c in OC specimens. Let-7c agomir was transfected into OC cells, and the proliferation and apoptosis of OC cells were detected. A dual-luciferase assay and western blotting were performed to analyze whether CDC25a was the target gene of let-7c as well as its interaction site. The results revealed that, in OC tissue, let-7c was downregulated when compared with normal ovarian tissue. A Cell Counting Kit-8 (CCK8) assay, colony formation assay and flow cytometry demonstrated that increased expression of let-7c was able to inhibit the proliferation and increase the apoptosis of OC cells. Western blotting revealed that upregulated let-7c is able to decrease the expression of CDC25a, and a dual-luciferase assay and a recovery assay demonstrated that let-7c was able to regulate the expression of the 3' untranslated region of CDC25a. Therefore, the roles of let-7c in inhibiting the proliferation and promoting the apoptosis of OC cells may be realized through the regulation of the expression of CDC25a. The results of the present study revealed that let-7c may be a novel target in the diagnosis and treatment of OC. |
Databáze: | OpenAIRE |
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