Growth hormone induces insulin-like growth factor-I gene transcription by a synergistic action of STAT5 and HNF-1α
| Autor: | John S. Sussenbach, Isidoro Metón, Elmieke P. J. Boot, P.H. Steenbergh |
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| Rok vydání: | 1999 |
| Předmět: |
Transcriptional Activation
medicine.medical_treatment Biophysics Transfection Biochemistry Insulin-like growth factor Genes Reporter Salmon Structural Biology Transcription (biology) STAT5 Transcription Factor Tumor Cells Cultured Genetics medicine Animals Hepatocyte Nuclear Factor 1-alpha Transcription activation Insulin-Like Growth Factor I Promoter Regions Genetic Molecular Biology Gene Transcription factor STAT5 Hepatocyte Nuclear Factor 1-beta biology Growth factor fungi Nuclear Proteins Receptors Somatotropin Cell Biology Milk Proteins Molecular biology IGF-I GH DNA-Binding Proteins HNF-1 Gene Expression Regulation Cell culture Growth Hormone Hepatocyte Nuclear Factor 1 Trans-Activators biology.protein Transcription Factors |
| Zdroj: | FEBS Letters. 444:155-159 |
| ISSN: | 0014-5793 |
| Popis: | Salmon insulin-like growth factor-I (sIGF-I) expression is, as in mammals, induced by growth hormone (GH). To elucidate the mechanism by which GH stimulates the transcription of the IGF-I gene, we transiently transfected Hep3B cells expressing the rat GH receptor with a sIGF-I promoter-luciferase reporter construct. Activation of the construct by GH added to the medium of the transfected cells was observed when two specific transcription factors, STAT5 and HNF-1α, were simultaneously overexpressed in these cells. This finding demonstrates for the first time a GH-dependent activation of an IGF-I promoter construct in an immortalized laboratory cell line. |
| Databáze: | OpenAIRE |
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