Correlation of promoter hypermethylation in hTERT, DAPK and MGMT genes with cervical oncogenesis progression
| Autor: | Ioannis E. Messinis, Aspasia Tsezou, Dimitrios Iliopoulos, Pagona Oikonomou |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Cancer Research Telomerase Adolescent Uterine Cervical Neoplasms Biology medicine.disease_cause Young Adult medicine Humans Telomerase reverse transcriptase Promoter Regions Genetic neoplasms DNA Modification Methylases Neoplasm Staging Cervical cancer Oncogene Tumor Suppressor Proteins Cancer General Medicine Methylation DNA Methylation Middle Aged medicine.disease Molecular biology Gene Expression Regulation Neoplastic Death-Associated Protein Kinases Cell Transformation Neoplastic DNA Repair Enzymes Oncology DNA methylation Calcium-Calmodulin-Dependent Protein Kinases Cancer research Disease Progression Female Carcinogenesis Apoptosis Regulatory Proteins Precancerous Conditions |
| Zdroj: | Oncology reports. 22(1) |
| ISSN: | 1021-335X |
| Popis: | DNA hypermethylation occurs during the multistep process of cervical carcinogenesis. We investigated whether the methylation status in the promoter region of a potential oncogene, the human telomerase reverse transcriptase (hTERT), and the tumor suppressor genes death-associated protein kinase (DAPK) and O 6 -methylguanine DNA methyltransferase (MGMT), were able to distinguish the early from late stages of cervical oncogenesis. The methylation status in the promoter of these genes was analyzed using real-time MethyLight analysis in 115 cervical specimens, including normal, premalignant [atypical squamous epithelial cells (ASCUS), low-grade squamous intraepithelial lesions (LGSIL), high-grade squamous intraepithelial lesions (HGSIL)] and cancer specimens. Clinicopathological parameters (cytology, histology, grade, stage) were compared to the levels of pro-moter hypermethylation. We found that hTERT, MGMT and DAPK hypermethylation levels were increased during cervical oncogenesis progression. hTERT promoter hypermethylation was able to distinguish normal from cancer (p=0.008), normal from premalignant (p=0.036), as well as premalignant from cervical cancer cases (p=0.003). A significant association was also observed between all three genes and the grade of cervical cancer, with hTERT showing a better association (p |
| Databáze: | OpenAIRE |
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