Effectiveness of ATP bioluminescence assay for presumptive identification of microorganisms in hospital water sources
| Autor: | Adriano Menis Ferreira, Marcelo Alessandro Rigotti, Natalia Seron Brizzotti, E. M. Castilho, Jacqueline Tanury Macruz Peresi, Oleci Pereira Frota, Margarete Teresa Gottardo de Almeida, Máira Gazzola Arroyo, Denise de Andrade |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Mato Grosso do Sul (UFMS), Universidade de São Paulo (USP), Fac Med Sao Jose do Rio Preto, Adolfo Lutz Inst |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microorganism 030106 microbiology Water source Heterotrophic bacteria Bacterial growth lcsh:Infectious and parasitic diseases Microbiology Hospital 03 medical and health sciences Adenosine Triphosphate ATP test Humans lcsh:RC109-216 Food science Bacteria biology Fungi Water FLUORESCÊNCIA biology.organism_classification Hospitals Atp bioluminescence Infectious Diseases Distilled water Luminescent Measurements Water Microbiology Brazil Research Article Adenosine triphosphate |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP BMC Infectious Diseases BMC Infectious Diseases, Vol 17, Iss 1, Pp 1-5 (2017) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1471-2334 |
| DOI: | 10.1186/s12879-017-2562-y |
| Popis: | Made available in DSpace on 2018-11-26T17:39:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-06-30 Background: Laboratory analysis of organisms in water include arduous methods, such as the multiple tube and membrane filter. The ATP bioluminescence system, proposes a new way of measuring cellular material in water by measuring adenosine triphosphate (ATP) levels, which are expressed in relative light units (RLU). The ATP bioluminescence assay has been increasingly used to assess the microbiological safety of the hospital environment. However, there are few studies investigating the use of this methodology to evaluate the microbiological quality of water. The objective of the present study was to verify whether ATP, as measured by the 3 M (TM) Clean-Trace Water (TM) ATP test, can be used as an alternative tool for presumptive testing for the presence of microorganisms in hospital water. Methods: Water samples (N = 88) were collected from faucets (74) and water purifiers (14) in a university hospital. The sample were filtered by the membrane filter technique (100 mL for bacterial analysis and 100 mL for fungal analysis) and then submitted to ATP bioluminescence assay to the determine quantity of RLU in each sample. In order to compare RLU and the presence of microorganisms, a receiver operating characteristic (ROC) curve was used to calculate sensitivity and specificity (levels higher than 90% were considered significant). In addition, control tests were conducted to compare RLU to the quantities of bacterial and fungal organisms added to distilled water (ANOVA and Tukey's tests; p |
| Databáze: | OpenAIRE |
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