Separation and cultivation of laryngeal carcinoma-associated fibroblasts and biological influence on a laryngeal carcinoma cell line

Autor:	Liang Zhou, Jie Tian, Chunping Wu, Huai-Dong Du
Rok vydání:	2013
Předmět:	Larynx Pathology medicine.medical_specialty Carcinoma associated fibroblasts Cell Survival Fluorescent Antibody Technique Cell Separation Biology Sampling Studies Carcinoma cell line Tissue culture Cell Movement Reference Values Carcinoma Cell Cell Line Tumor medicine Conditioned medium Tumor Microenvironment Humans Laryngeal Neoplasms Cell Proliferation General Medicine Fibroblasts Immunohistochemistry medicine.anatomical_structure Otorhinolaryngology Culture Media Conditioned Carcinoma Squamous Cell Positive staining
Zdroj:	Acta oto-laryngologica. 133(7)
ISSN:	1651-2251
Popis:	Carcinoma-associated fibroblasts (CAFs) can influence the biological characteristics of a laryngeal carcinoma cell line. These results could lay the foundation for further studies on the role of CAFs in the laryngeal tumor-host microenvironment.CAFs are important contributors to the microenvironment in determining the fate of tumors. The aim of this study was to separate, culture, and identify laryngeal CAFs and investigate their biological influence on the laryngeal carcinoma cell line.The primary CAFs and normal fibroblasts (NFs) of the larynx were obtained by tissue culture. The cells were verified according to immunohistochemical and immunofluorescence staining of certain proteins. Conditioned medium (CM) from CAFs and NFs was obtained. Functional assays were performed to test the influence of each CM on laryngeal carcinoma cell lines.Third-passage purified laryngeal CAFs and NFs were successfully attained. The CAFs showed positive staining for vimentin, α-smooth muscle actin (α-SMA), and fibroblast activation protein (FAP). The migration ability of the CAFs increased significantly compared with that of NFs (p0.05). CM from CAFs (compared with CM from NFs) stimulated proliferation, migration, and invasion to a greater extent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::212b495331f4b7c77318b6426f52d0ca https://pubmed.ncbi.nlm.nih.gov/23448354 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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