Affinity chromatography on columns containing nucleic acids
| Autor: | Hans Potuzak, Peter D.G. Dean |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Ultraviolet Rays
Biophysics Biochemistry Chromatography Affinity Phosphates Sepharose chemistry.chemical_compound Affinity chromatography Structural Biology Polysaccharides Genetics Ultraviolet light Nucleotide Cyanogen Bromide Cellulose Molecular Biology chemistry.chemical_classification Chromatography Oligonucleotide Cell Biology DNA chemistry Polynucleotide Nucleic acid Epoxy Compounds Adsorption Carrier Proteins |
| Zdroj: | FEBS Letters. (2):161-166 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(78)80165-3 |
| Popis: | Chromatography on affinity adsorbents containing polynucleotide ligands dates back to 1968 when successful purification of micrococcal DNA poly- merase on a column containing DNA immobilized onto cellulose after irradiation with ultraviolet light was described by Rose Litman [1]. In the same year both Bruce Alberts [2] and Peter Gilham [3] independently suggested new methods for the prep- aration of adsorbents containing nucleic acids follow- ing earlier attempts mainly with oligonucleotides [4,5] or heavily damaged DNA [6,7] linked to different insoluble supports. Since that time, affinity chro- matography using immobilized nucleic acids has established itself as a useful technique [8-10] which has recently received diverse apphcations in molec- ular biology [11-20]. The following discussion summarizes the most significant methods of immobilization of poly- nucleotides and analyses applications of the affinity adsorbents obtained. |
| Databáze: | OpenAIRE |
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