Regulation of Proliferation by Fibroblast Growth Factor in a Pancreatic Cancer Cell Line
| Autor: | L. De Vries, J. P. Tauber, Nicole Vaysse, F. Prats, N. Tahiri-Jouti, Christiane Susini, Nathalie Viguerie, Lucien Pradayrol, Jean-Luc Scemama, M. Bensaïd |
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| Rok vydání: | 1990 |
| Předmět: |
Cell type
Cell growth Basic fibroblast growth factor Gastroenterology Fibroblast growth factor receptor 3 Biology Ornithine Decarboxylase Fibroblast growth factor Molecular biology Cell Line Rats Cell biology Ornithine decarboxylase Fibroblast Growth Factors Pancreatic Neoplasms chemistry.chemical_compound chemistry Cell culture Tumor Cells Cultured Animals Cell Division Intracellular |
| Zdroj: | Digestion. 46:162-165 |
| ISSN: | 1421-9867 0012-2823 |
| Popis: | Basic fibroblast growth factor (bFGF) is a potent mitogen for various cell types. We report here the first study of the effects of bFGF on a digestive tract-derived cell line. The effect of bFGF on the proliferation of AR4-2J cells, tumor cells of acinar pancreatic origin, was investigated together with modulation of ornithine decarboxylase (ODC) activity, an intracellular event involved in cell proliferation. bFGF caused a concentration-dependent stimulation of AR4-2J cell growth, with a half maximal effect (EC50) at 22 +/- 2 pM. ODC activity, assayed by the CO2-trapping method, was also increased by bFGF in a dose-dependent manner, reaching half-maximal stimulation at 20 pM. We conclude that bFGF is a very potent growth promoting factor for cells of pancreatic origin, already effective at picomolar concentrations. The parallelism between the growth assay and the ODC activity assay implicates the involvement of ODC activity in the pathway of the mitogenic effect of bFGF. The stimulation of ODC activity therefore seems to be a reliable early marker for cell proliferation in this model. |
| Databáze: | OpenAIRE |
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