Excretion of metabolites of the synthetic cannabinoid JWH-018 in urine after controlled inhalation

Autor: Werner Pogoda, Alexander Paulke, Stefan W. Toennes, Johannes G. Ramaekers, Anna Geraths, Eef L. Theunissen, Cora Wunder
Přispěvatelé: Section Psychopharmacology, RS: FPN NPPP II
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Indoles
Synthetic cannabinoids
Metabolite
Clinical Biochemistry
Pharmaceutical Science
Pilot Projects
Urine
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
0302 clinical medicine
Tandem Mass Spectrometry
Drug Discovery
Naphthalenes/administration & dosage
JWH-018
Spectroscopy
Biotransformation
Cannabinoids/administration & dosage
Inhalation Exposure
Chromatography
Liquid
Cross-Over Studies
Inhalation
medicine.diagnostic_test
Biomarkers/urine
Pentanoic Acids/urine
Smoking
QUANTITATIVE MEASUREMENT
Indoles/administration & dosage
Smoking
Non-Tobacco Products
Butyrates
Female
Butyrates/urine
medicine.drug
Urinalysis
Naphthalenes
Non-Tobacco Products
Hydroxylation
Excretion
03 medical and health sciences
Young Adult
Pharmacokinetics
medicine
MS/MS
Humans
030216 legal & forensic medicine
TANDEM MASS-SPECTROMETRY
Pentanoic Acids
Liquid chromatography-mass spectrometry
Cannabinoids
010401 analytical chemistry
SPECIMENS
0104 chemical sciences
Renal Elimination
Metabolism
chemistry
Biomarkers
Chromatography
Liquid
Zdroj: Journal of Pharmaceutical and Biomedical Analysis, 150, 162-168. Elsevier
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2017.12.016
Popis: Each year, synthetic cannabinoids are occurring in high numbers on the illicit drug market but data obtained after controlled application are rare. The present study on pharmacokinetics in urine is part of a pilot study on adverse effects of JWH-018, which is one of the oldest and best known synthetic cannabinoids. Six subjects inhaled smoke from 2 and 3mg JWH-018. The drug and ten potential metabolites were analyzed in urine samples collected during 12h after inhalation by liquid chromatography-mass spectrometry (LC-MS/MS) without and with conjugate cleavage. The parent compound was not detectable, but 13 of its metabolites, all of which were conjugated. Concentrations of the predominant metabolite, JWH-018 pentanoic acid, were less than 5ng/ml, but in two subjects it was still detected up to 4 weeks after ingestion. Other major metabolites were 5- and 4-HOpentyl-JWH-018, JWH-073 butanoic acid and a hypothetically dihydroxylated and dehydrogenated metabolite of JWH-018. Occasionally, further hydroxylated metabolites were found. Generally, hydroxylated metabolites were detected in concentrations lower than 1ng/ml already 10h after inhalation. All concentrations were much lower than reported for urine samples of authentic JWH-018 users. The formation of the metabolite JWH-018 pentanoic acid was found to be slightly delayed, but its rather high concentrations and detection over several weeks after single dosing makes it a useful target for urine analysis. The different excretion of carboxylic acid and hydroxylated metabolites may aid in evaluation of time of use.
Databáze: OpenAIRE
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