Some characteristics of Ureaplasma urealyticum. Urease activity in a simple buffer: effect of metal ions and sulphydryl inhibitors
Autor: | Leonard Hayflick, Gerald K. Masover, Stephen L. Swanberg |
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Rok vydání: | 1978 |
Předmět: |
Lysis
Urease Metal ions in aqueous solution Inorganic chemistry medicine.disease_cause Microbiology Ureaplasma chemistry.chemical_compound medicine Urea Edetic Acid HEPES biology Sulfhydryl Reagents Hydrogen-Ion Concentration biology.organism_classification Culture Media Digitonin chemistry biology.protein Nuclear chemistry Ureaplasma urealyticum |
Zdroj: | Journal of general microbiology. 108(2) |
ISSN: | 0022-1287 |
Popis: | Urealytic activity of the cytoplasmic fraction of Ureaplasma urealyticum prepared by digitonin lysis was assayed in a simple buffer system (HEPES plus EDTA) by measuring the release of 14CO2 from [14C]urea. The Km of this preparation agreed with our previous observations of the same activity measured in a more complex reaction mixture. The substrate concentration at which maximum velocity occurred was approximately 20 mM. The activity was sensitive to heavy metals and inhibitors which react with sulphydryl groups such as N-ethylmaleimide and p-chloromercuribenzoate. It was not inhibited by Ca2+ or Mg2+ or by the reaction products, ammonia and carbon dioxide. |
Databáze: | OpenAIRE |
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