Skewed T cell receptor Vα repertoire among superantigen reactive murine T cells
| Autor: | H. Robson MacDonald, A R Lussow, Gary A. Waanders |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Genetically modified mouse
Staphylococcus aureus CD3 Complex Hydrocortisone CD8 Antigens Receptors Antigen T-Cell alpha-beta Lymphocyte Immunology Mice Inbred Strains Mice Transgenic chemical and pharmacologic phenomena Immunogenetics Biology Lymphocyte Activation Clonal deletion Minor Lymphocyte Stimulatory Antigens Enterotoxins Mice T-Lymphocyte Subsets medicine Superantigen Animals Immunology and Allergy Cells Cultured Antigens Bacterial T-cell receptor hemic and immune systems General Medicine T lymphocyte Flow Cytometry Molecular biology In vitro medicine.anatomical_structure CD4 Antigens |
| Zdroj: | International Immunology. 5:55-61 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/5.1.55 |
| Popis: | Reactivity of murine T cells with viral or bacterial superantigens is clearly correlated with the expression of TCR V beta domains. Thus, T cells responding to the minor lymphocyte stimulatory locus (Mls-1a) or staphylococcal enterotoxin B (SEB) express predominantly TCR V beta 6 or V beta 8.2 respectively. We have investigated the involvement of the other major variable element of the TCR, the V alpha domain, in these superantigen responses. Using a panel of anti-TCR V alpha mAbs, it is demonstrated that the TCR V alpha repertoire among superantigen stimulated V beta 6+ or V beta 8.2+ blasts (responding to Mls-1a or SEB respectively in vitro) is altered in comparison with anti-CD3 stimulated cells expressing the same V beta domains. Furthermore, the TCR V alpha repertoire is strongly skewed in TCR V beta 8.2 transgenic mice that have undergone extensive peripheral clonal deletion after SEB injection. These data imply that the V alpha domain influences superantigen recognition by the TCR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|