Polarized Raman microspectroscopy can reveal structural changes of peritumoral dermis in basal cell carcinoma
| Autor: | Elodie Ly, Anne Durlach, Michel Manfait, Olivier Piot, Philippe Bernard |
|---|---|
| Přispěvatelé: | Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2008 |
| Předmět: |
Skin Neoplasms
Analytical chemistry Human skin Spectrum Analysis Raman Sensitivity and Specificity 030207 dermatology & venereal diseases 03 medical and health sciences symbols.namesake 0302 clinical medicine Nuclear magnetic resonance Dermis Microscopy medicine Humans Basal cell carcinoma Instrumentation ComputingMilieux_MISCELLANEOUS Spectroscopy 030304 developmental biology 0303 health sciences Chemistry Reproducibility of Results medicine.disease Raman microspectroscopy medicine.anatomical_structure Carcinoma Basal Cell symbols Epidermis Microscopy Polarization Raman spectroscopy Molecular probe |
| Zdroj: | Applied Spectroscopy Applied Spectroscopy, Society for Applied Spectroscopy, 2008, 62 (10), pp.1088-1094 |
| ISSN: | 0003-7028 |
| Popis: | Polarized Raman microspectroscopy can provide precious information regarding the orientation and ordering of the molecules in a sample without staining or particular preparation. This technique is used for the first time on a human skin section to probe the molecular modifications of the surrounding dermis in superficial basal cell carcinoma. Spectra using polarized and conventional Raman microspectroscopies were recorded on dermis bordering either the tumor or healthy epidermis. Band areas and spectral decomposition on selected vibrations were computed. Significant differences in dermal collagen vibration bands are detected using both polarized and conventional micro-spectroscopies, but the spectral changes between tumor and healthy tissues are enhanced using polarized Raman microspectroscopy. The analysis of these spectral differences highlights structural modifications of the triple helix of collagen. We see polarized Raman microspectroscopy as a potential tool that could be implemented for clinical analyses to guide clinicians and surgeons in the treatment of aggressive skin cancers. The information obtainable could also help better elucidate the molecular mechanisms induced in basal cell carcinoma development. |
| Databáze: | OpenAIRE |
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