Lack of evidence for the prodection of interreron -α-like species by the cultured human pre-embryo
| Autor: | Tim Chard, Sheryl T. Homa, M.J. Searle, Lionel K. Gunn |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Alpha interferon Fertilization in Vitro Biology Chorionic Gonadotropin Andrology Pregnancy Culture Techniques Internal medicine medicine Humans Blastocyst reproductive and urinary physiology Interferon alfa urogenital system Rehabilitation Interferon-alpha Obstetrics and Gynecology Trophoblast Embryo culture Embryo Radioimmunoassay Endocrinology medicine.anatomical_structure Reproductive Medicine embryonic structures Female Gonadotropin medicine.drug |
| Zdroj: | Human Reproduction. 9:1522-1527 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/oxfordjournals.humrep.a138742 |
| Popis: | The aim of this study was to determine if the human pre-embryo produces a substance similar to the trophoblast interferon secreted by ruminant trophoblasts. Human embryos surplus to in-vitro fertilization (IVF) treatment were cultured up to 14 days following IVF. Viable cultures were determined by microscopic examination and by assay of the culture medium for human chorionic gonadotrophin (HCG). Four stages of development were visually identified: pre-blastocyst, unhatched, part-hatched and fully hatched blastocyst. HCG was detected in medium which had contained the more developmentally advanced embryos. A total of 62 samples were assayed for human interferon-alpha (IFN-alpha), including all cultures presumed viable. None contained detectable IFN-alpha immunoreactivity. Out of 14 candidate samples subjected to cytopathic effect reduction assay, none contained antiviral activity. We suggest that a trophoblast-derived interferon, unlike HCG, does not play a significant role in the maternal recognition of pregnancy in humans. |
| Databáze: | OpenAIRE |
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