Size distribution of rhodamine-labelled microspheres retrogradely transported in cultured neurons
| Autor: | Horstmar Holländer, G. Dirlich, Rupert Egensperger |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Mean diameter
education.field_of_study Ganglia Sympathetic Rhodamines General Neuroscience Population Anatomy Biology Axonal Transport Retrograde tracing Microspheres Rats Microsphere Rhodamine chemistry.chemical_compound Xanthenes chemistry Permeability (electromagnetism) Axoplasmic transport Biophysics Animals education Cells Cultured Fluorescent Dyes |
| Zdroj: | Journal of Neuroscience Methods. 29:1-4 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/0165-0270(89)90102-7 |
| Popis: | Rhodamine-labelled latex microspheres (RLM), 10–110 nm in diameter, were used as a retrograde tracer in cultured sympathetic neurons. Retrogradely transported RLM were isolated from the cells and measured. These were all smaller than 50 nm in diameter and represented not more than 2% of the original RLM population. The permeability of RLM as a function of size was also estimated and showed a steep decrease between 20 and 30 nm from 100% to less than 20%. Therefore, RLM with a mean diameter of 20 nm would be optimal for retrograde tracing. |
| Databáze: | OpenAIRE |
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