MOESM1 of Transcriptome and gene expression analysis of docosahexaenoic acid producer Schizochytrium sp. under different oxygen supply conditions

Autor: Bi, Zhi-Qian, Lu-Jing Ren, Xue-Chao Hu, Sun, Xiao-Man, Zhu, Si-Yu, Ji, Xiao-Jun, Huang, He
Rok vydání: 2018
DOI: 10.6084/m9.figshare.7097051
Popis: Additional file 1: Figure S1. Summary of draft reads of samples by Illumina deep sequencing. (A) Distribution of number of unique reads. (B) Global comparison of normal-oxygen sample and high oxygen sample by Venn diagrams. (C) Number of upregulated and down-regulated DEGs of the normal-oxygen and high oxygen samples. Figure S2. Gene ontology (GO) functional analysis of unique sequences from normal and high oxygen transcriptome. Unique sequences were assigned to three categories: molecular functional, cellular components, and biological process. Figure S3. KEGG pathway enrichment of assembled unigenes. Rich Factor: the number of DEGs in specific pathway term/the number of all genes in specific pathway term. Gene Number: the number of DEGs in specific pathway. Q value: False discovering rate. Pathways with Q value â ¤ 0.05 are significantly enriched in DEGs. Figure S4. Real-time quantitative PCR results for the FAS, ORFA, ORFB, ORFC, ACC, and ME genes from the Schizochytrium sp. HX-308. Values and error bars represent the means and the standard deviations of triplicate experiments. Table S1. The gene information list mentioned in this paper. Table S2. Primers for genes validated by Quantitative real-time PCR (qPCR).
Databáze: OpenAIRE