Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer
| Autor: | Shijun Li, Gong Cheng, Linsen Zan, C.Z. Fu, Bijie Jiang, Shengjuan Wei, Camus Adoligbe, Hong Wang, Hongbao Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Transgene
Genetic Vectors Bioengineering Beef cattle Applied Microbiology and Biotechnology Animals Genetically Modified Fatty Acids Omega-3 Animals Caenorhabditis elegans Gene chemistry.chemical_classification biology food and beverages General Medicine biology.organism_classification eye diseases Genetically modified organism Red Meat Fatty acid desaturase Biochemistry chemistry biology.protein Somatic cell nuclear transfer Cattle lipids (amino acids peptides and proteins) Plasmids Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Biotechnology Letters. 37:1565-1571 |
| ISSN: | 1573-6776 0141-5492 |
| Popis: | Omega-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial to human health. However, the n-3 PUFAs contents of the livestock meat that we consume daily are relatively low. Utilization of transgenic technology to increase n-3 PUFAs contents in livestock may solve this problem. The omega-3 fatty acid desaturase (FAD3), encoded by fat1 gene derived from Caenorhabditis elegans (C. elegans), converts omega-6 polyunsaturated fatty acids (n-6 PUFAs) to n-3 PUFAs. In the study, a plasmid containing the codon-optimized C. elegans fat1 gene (mfat1) was constructed and used to produce transgenic beef cattle by somatic cell nuclear transfer. Fourteen transgenic calves were obtained, and the ratio of n-6 to n-3 PUFAs in the transgenic calves decreased from 5.33: 1 to 0.95: 1 compared with negative controls. Our results demonstrated that the codon-optimized C. elegans mfat1 gene can be functionally expressed in the beef cattle and converts n-6 PUFAs to n-3 PUFAs. |
| Databáze: | OpenAIRE |
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