Undeniable Evidence That the Adult Mammalian Heart Lacks an Endogenous Regenerative Stem Cell
| Autor: | Bryan D. Maliken, Jeffery D. Molkentin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Adult 0301 basic medicine Mice 129 Strain Myocardial Infarction Mice Transgenic Endogeny Article Recombinases Mice 03 medical and health sciences 0302 clinical medicine Physiology (medical) Regeneration Animals Medicine Myocytes Cardiac Cell Lineage Muscle Skeletal Cell Proliferation Integrases business.industry Escherichia coli Proteins Stem Cells Gene Expression Regulation Developmental Cell Differentiation Mouse Embryonic Stem Cells Heart Mammalian heart Cell biology Mice Inbred C57BL Adult Stem Cells Disease Models Animal Phenotype 030104 developmental biology Cell Tracking Female Stem cell Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Circulation. 138:806-808 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/circulationaha.118.035186 |
| Popis: | Whether the adult mammalian heart harbors cardiac stem cells for regeneration of cardiomyocytes is an important yet contentious topic in the field of cardiovascular regeneration. The putative myocyte stem cell populations recognized without specific cell markers, such as the cardiosphere-derived cells, or with markers such as Sca1To address this question without relying on a particular stem cell marker, we developed a new genetic lineage tracing system to label all nonmyocyte populations that contain putative cardiac stem cells. Using dual lineage tracing system, we assessed whether nonmyocytes generated any new myocytes during embryonic development, during adult homeostasis, and after myocardial infarction. Skeletal muscle was also examined after injury for internal control of new myocyte generation from nonmyocytes.By this stem cell marker-free and dual recombinases-mediated cell tracking approach, our fate mapping data show that new myocytes arise from nonmyocytes in the embryonic heart, but not in the adult heart during homeostasis or after myocardial infarction. As positive control, our lineage tracing system detected new myocytes derived from nonmyocytes in the skeletal muscle after injury.This study provides in vivo genetic evidence for nonmyocyte to myocyte conversion in embryonic but not adult heart, arguing again the myogenic potential of putative stem cell populations for cardiac regeneration in the adult stage. This study also provides a new genetic strategy to identify endogenous stem cells, if any, in other organ systems for tissue repair and regeneration. |
| Databáze: | OpenAIRE |
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