mAngiogenin-3, a target gene of oncoprotein E2a-Pbx1, encodes a new angiogenic member of the angiogenin family
| Autor: | Shapiro R, Mark P. Kamps, Nobile, Fu X, Roberts Wg |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Angiogenin
Oncogene Proteins Fusion Angiogenesis medicine.medical_treatment Clinical Biochemistry Basic fibroblast growth factor Molecular Sequence Data Neovascularization Physiologic Chick Embryo Biology 3T3 cells chemistry.chemical_compound Mice Endocrinology Ribonucleases medicine Animals Amino Acid Sequence Muscle Skeletal Homeodomain Proteins Base Sequence Growth factor Endoplasmic reticulum Cell Biology 3T3 Cells Molecular biology Rats Vascular endothelial growth factor Microscopy Electron medicine.anatomical_structure chemistry biology.protein Pancreatic ribonuclease |
| Zdroj: | Growth factors (Chur, Switzerland). 17(2) |
| ISSN: | 0897-7194 |
| Popis: | Angiogenins are proteins in the pancreatic ribonuclease superfamily that utilize their ribonuclease activity to induce formation of new blood vessels. Recently we identified a new member of the angiogenin gene family, mouse angiogenin-3, by virtue of its transcriptional activation in NIH3T3 fibroblasts coincident with transformation by the chimeric leukemia oncogene, E2a-Pbx1. Here we have isolated the cDNA encoding mouse angiogenin-3 and used it to produce the protein in E. coli. We demonstrate that mouse angiogenin-3 is a ribonuclease whose activity and specificity towards tRNA and dinucleotide substrates differ from those of mouse angiogenin or of mouse angiogenin-related protein, a non-angiogenic factor. Mouse angiogenin-3 induced angiogenesis in both the chicken embryo chorioallantoic membrane assay and the rat cremaster muscle. Electron microscopy revealed that endothelial cells within vessels induced by both mouse angiogenin-3 and mouse angiogenin contain fenestrations similar to those observed in endothelial cells from neovasculature induced by vascular endothelial growth factor and basic fibroblast growth factor. Mouse angiogenin-3 also induced other molecular events typical of rapidly proliferating endothelial cells, such as increases in rough endoplasmic reticulum, polysomes, and mitochondria. |
| Databáze: | OpenAIRE |
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