Ribosomes initiating translation of the hbs mRNA protect it from 5'-to-3' exoribonucleolytic degradation by RNase J1
| Autor: | Roula Daou-Chabo, Lionel Benard, Nathalie Mathy, Ciarán Condon |
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| Přispěvatelé: | Expression Génétique Microbienne (EGM (UMR_8261 / FRE_3630)), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Régulation de l'expression génétique chez les microorganismes (REGCM), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Gauthier, Laurence, Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Exonuclease
RNA Stability RNase P [SDV]Life Sciences [q-bio] Microbiology 03 medical and health sciences Bacterial Proteins [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Protein biosynthesis [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Ribonuclease Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Messenger RNA biology [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] 030306 microbiology Gene Expression Regulation Bacterial Genetic translation DNA-Binding Proteins RNA Bacterial RNase MRP Biochemistry Protein Biosynthesis Exoribonucleases biology.protein Nucleic Acid Conformation Ribosomes Bacillus subtilis |
| Zdroj: | Molecular Microbiology Molecular Microbiology, Wiley, 2009 Molecular Microbiology, Wiley, 2009, 71, pp.1538-1550 Molecular Microbiology, Wiley, 2009, 71 (6), pp.1538-1550. ⟨10.1111/j.1365-2958.2009.06620.x⟩ Molecular Microbiology, 2009, 71 (6), pp.1538-1550. ⟨10.1111/j.1365-2958.2009.06620.x⟩ |
| ISSN: | 0950-382X 1365-2958 |
| DOI: | 10.1111/j.1365-2958.2009.06620.x⟩ |
| Popis: | The discovery of the essential ribonuclease RNase J1, involved in global mRNA decay in Bacillus subtilis, has paved the way for studies on the turnover pathways of specific RNAs in this organism. Here we report an effect of RNase J1 depletion on both the maturation and degradation of the hbs mRNA, encoding the B. subtilis orthologue of the histone-like protein HU. The major hbs transcript observed in wild-type cells is generated by the blocking of 5'-to-3' exonuclease activity of RNase J1 by ribosomes initiating translation of this mRNA. Increasing the strength of the Shine-Dalgarno (SD) sequence leads to greater accumulation of this species, while weakening of the SD leads to its disappearance. The 5'-to-3' exonuclease activity of RNase J1 is also required for the turnover of the hbs mRNA, specifically the 3' half of the transcript. For both the maturation and degradation reactions, RNase J1 access to the mRNA requires prior endonucleolytic cleavage. |
| Databáze: | OpenAIRE |
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