Non-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment
| Autor: | Ka-Fai Leung, Carol Wai-Kwan Siu, Wing-Kin To, Wing-Tat Poon, Ching-Wan Lam, KC Lau, Paul K.S. Chan, Sik To Lai, Yan-Wo Chan, Chloe Miu Mak, Tak-Yin Tsang, Chun-Yiu Law |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Hepatitis B virus Hepatitis B vaccine Clinical Biochemistry Human leukocyte antigen HLA-DP alpha-Chains medicine.disease_cause Major histocompatibility complex Biochemistry Young Adult Hepatitis B Chronic medicine Humans Genetic Testing Precision Medicine Genotyping Alleles HLA-DP beta-Chains Aged Aged 80 and over biology HLA-DPB1 Transmission (medicine) Biochemistry (medical) Vaccination General Medicine Hepatitis B Middle Aged medicine.disease Virology Infectious Disease Transmission Vertical Genetic Loci Immunology biology.protein Female |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 412(11-12) |
| ISSN: | 1873-3492 |
| Popis: | Polymorphisms in the major histocompatibility complex (MHC) and non-MHC genes were recently reported to be associated with persistent hepatitis B virus (HBV) infection and host response to hepatitis B vaccine in Asian populations. We aimed to confirm the associations in Chinese population and develop a non-invasive screening method for the risk loci.We genotyped 2 risk alleles on the MHC loci, HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277535), and 1 risk allele near a non-MHC gene, FOXP1 (rs6789153) using high-resolution melting curve analysis. With minimal processing steps and time, salivary DNA was extracted with a modified protocol of a blood kit. We compared the genotyping fidelity between peripheral blood DNA and salivary DNA.Both rs3077 and rs9277535, but not rs6789153, are significantly associated with CHB in Chinese population (p-value0.001). High genotype concordance between different sources of genomic DNA was obtained.Genotyping salivary DNA using our modified methods provides a non-invasive fast screening for host susceptibility loci. The transmission mechanism of hepatitis B can now be modified by adding genetic susceptibility to the traditional vertical transmission model of hepatitis B. |
| Databáze: | OpenAIRE |
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