Fast Morphological Gallbladder Changes Triggered by a Hypercholesterolemic Diet
| Autor: | Karina Martínez-Flores, Gilberto Vargas-Alarcón, Denise Clavijo-Cornejo, Luis Alonso Morales-Garza, Erendida G Estrada-Villaseñor, Daniel Medina-Luna, Julio Granados, Luis Enrique Gómez-Quiroz, Hiram García, Alberto López-Reyes, Marwin Gutierrez, Carlos Pineda, María Concepción Gutiérrez-Ruiz, Javier Fernández-Torres |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Time Factors Hypercholesterolemia Histopathology Specialties of internal medicine Physiology Gallstones Lithiasis Gallbladder Stone Hepatic Diseases Cholesterol Dietary 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ultrasound medicine Animals Bile Risk factor Ultrasonography Hepatology Cholesterol business.industry Gallbladder Fatty liver General Medicine medicine.disease Obesity Fatty Liver Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure RC581-951 chemistry 030211 gastroenterology & hepatology Microscopy Polarization Crystallization business |
| Zdroj: | Annals of Hepatology, Vol 17, Iss 5, Pp 857-863 (2018) |
| ISSN: | 1665-2681 |
| DOI: | 10.5604/01.3001.0012.3160 |
| Popis: | Introduction and aim. Obesity is a worldwide epidemic problem, described as a risk factor for hepatic diseases, such as non-alcoholic fatty liver disease and other pathologies related to development of cholesterol crystals and cholesterol gallbladder stones. It has been reported that cholesterol overload may cause hepatic damage; however, little is known about the effects of an acute hypercholesterolemic diet on the gallbladder. The aim of this manuscript was to evaluate the impact of a cholesterol-rich diet on the gallbladder. Material and methods. The study included ten eight-week-old C57BL6 male mice, which were divided into two study groups and fed different diets for 48 h: a hypercholesterolemic diet and a balanced Chow diet. After 48 h, the mice were analyzed by US with a Siemens Acuson Antares equipment. Mice were subsequently sacrificed to carry out a cholesterol analysis with a Refloton System (Roche), a crystal analysis with a Carl Zeiss microscope with polarized light, and a histological analysis with Hematoxylin-eosin staining. Results. The hypercholesterolemic diet induced an increase in gallbladder size and total cholesterol content in the bile, along with important histological changes. Conclusion. Cholesterol overloads not only trigger hepatic damage, but also affect the gallbladder significantly. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|