Spliced leader RNAs from lower eukaryotes are trans- spliced in mammalian cells
| Autor: | James P. Bruzik, Tom Maniatis |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
RNA Splicing
Genetic Vectors Molecular Sequence Data Restriction Mapping Transfection Euglena Cell Line Exon In vivo RNA Small Nuclear Animals Humans splice RNA Messenger Caenorhabditis elegans Cell Nucleus Multidisciplinary Base Sequence biology fungi RNA Exons biology.organism_classification Actins In vitro Cell biology Oligodeoxyribonucleotides RNA splicing Spliced Leader RNA Trypanosomatina HeLa Cells |
| Zdroj: | Nature. 360:692-695 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/360692a0 |
| Popis: | EXON sequences present on separate RNA molecules can be joined by trans-splicing in trypanosomatids, Euglena, and in the nematode and trematode worms1–3. Trans-splicing involves an interaction between a 5′ splice site present in a spliced leader RNA and a 3′ splice site located near the 5′ end of pre-messenger RNAs. In vitro trans-splicing of artificial mammalian pre-mRNAs has been reported, but the efficiency of splicing appears to depend on sequence complementarity between the two substrates4–7. There has been speculation that some natural pre-mRNAs can be trans-spliced in mammalian cells in vivo8,9, but alternative interpretations have not been ruled out. Here we show that spliced leader RNAs can be accurately trans-spliced in mammalian cells in vivo and in vitro. Both nematode and mammalian 3′ splice sites can function as acceptors for trans-splicing in vivo. These results reveal functional conservation in the splicing machinery between lower eukaryotes and mammals, and they directly demonstrate the potential for trans-splicing in mammalian cells. |
| Databáze: | OpenAIRE |
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