Evolutionary study of duplications of the miRNA machinery in aphids associated with striking rate acceleration and changes in expression profiles

Autor: Sylvie Tanguy, Denis Tagu, Benjamín Ortiz-Rivas, Jean-Pierre Gauthier, Stéphanie Jaubert-Possamai, Rispe Claude
Přispěvatelé: Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
0106 biological sciences
phylogénétique
Male
Ribonuclease III
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Subfamily
Gene duplication
01 natural sciences
Genome
duplication de gènes
pcr semi quantitative
Phylogeny
Regulation of gene expression
Genetics
0303 health sciences
biology
Reverse Transcriptase Polymerase Chain Reaction
Reproduction
food and beverages
Agricultural sciences
puceron
Argonaute Proteins
miRNAs
Insect Proteins
Neofunctionalization
Female
Research Article
Evolution
plasticité phénotypique
Molecular Sequence Data
Gene Conversion
acyrthosiphon pisum
010603 evolutionary biology
Evolution
Molecular
03 medical and health sciences
analyse de génome
QH359-425
Animals
Gene
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Models
Genetic
Gene Expression Profiling
Sequence Analysis
DNA
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Acyrthosiphon pisum
Gene expression profiling
Alternative Splicing
MicroRNAs
Aphids
Sciences agricoles
Zdroj: BMC Evolutionary Biology
BMC Evolutionary Biology, BioMed Central, 2012, 12, pp.216. ⟨10.1186/1471-2148-12-216⟩
BMC Evolutionary Biology, 2012, 12, pp.216. ⟨10.1186/1471-2148-12-216⟩
BMC Evolutionary Biology (12), 216. (2012)
BMC Evolutionary Biology, Vol 12, Iss 1, p 216 (2012)
ISSN: 1471-2148
Popis: The sequencing of the genome of the pea aphid Acyrthosiphon pisum revealed an unusual expansion of the miRNA machinery, with two argonaute-1, two dicer-1 and four pasha gene copies. In this report, we have undertaken a deeper evolutionary analysis of the phylogenetic timing of these gene duplications and of the associated selective pressures by sequencing the two copies of ago-1 and dcr-1 in different aphid species of the subfamily Aphidinae. We have also carried out an analysis of the expression of both copies of ago-1 and dcr-1 by semi-quantitative PCR in different morphs of the pea aphid life cycle.
The analysis has shown that the duplication of ago-1 occurred in an ancestor of the subfamily Aphidinae while the duplication of dcr-1 appears to be more recent. Besides, it has confirmed a pattern of one conserved copy and one accelerated copy for both genes, and has revealed the action of positive selection on several regions of the fast-evolving ago-1b. On the other hand, the semi-quantitative PCR experiments have revealed a differential expression of these genes between the morphs of the parthenogenetic and the sexual phases of Acyrthosiphon pisum.
The discovery of these gene duplications in the miRNA machinery of aphids opens new perspectives of research about the regulation of gene expression in these insects. Accelerated evolution, positive selection and differential expression affecting some of the copies of these genes suggests the possibility of a neofunctionalization of these duplicates, which might play a role in the display of the striking phenotypic plasticity of aphids.
Databáze: OpenAIRE
Externí odkaz: