Effect of sodium fluoride on male mouse fertility

Autor:	Myung-Geol Pang, June Sub Lee, Saidur Rahman, Jin Kim, Young Ah You, Woo-Sung Kwon, Yoo-Jin Park, Sung Jae Yoon
Rok vydání:	2015
Předmět:	Male inorganic chemicals Urology Endocrinology Diabetes and Metabolism Acrosome reaction Embryonic Development Motility Fertilization in Vitro Biology Andrology Mice chemistry.chemical_compound Adenosine Triphosphate Endocrinology Human fertilization Capacitation Sodium fluoride medicine Animals Blastocyst Infertility Male Sperm motility Acrosome Reaction technology industry and agriculture Cyclic AMP-Dependent Protein Kinases Spermatozoa Sperm body regions medicine.anatomical_structure Reproductive Medicine chemistry Fertilization Sperm Motility Sodium Fluoride Calcium Female Sperm Capacitation
Zdroj:	Andrology. 3:544-551
ISSN:	2047-2919
DOI:	10.1111/andr.12006
Popis:	Sodium fluoride (NaF), an environmental pollutant, has been tested for its impact on fertility in several species of laboratory animals. A literature demonstrated that NaF adversely affects sperm motility, morphology, capacitation, and the acrosome reaction. However, the molecular mechanisms underlying these alterations have not yet been elucidated. Therefore, present study was designed to evaluate the regulatory pathways involved in the effect of NaF on sperm function and fertilization. In this in vitro study, mouse spermatozoa were incubated with a range of concentrations (2.5, 5, and 10 mm) of NaF for 90 min in media that support in vitro fertilization. Our results showed that NaF was associated with reduced intracellular ATP generation, motility, and motion kinematics. Likewise, short-term exposure of spermatozoa to NaF significantly reduced the intracellular calcium concentration, protein kinase-A activity, and tyrosine phosphorylation of sperm proteins, which were associated with a significant decrease in the rate of capacitation and the acrosome reaction. Finally, NaF significantly reduced the fertilization and blastocyst formation during early embryonic development. On the basis of these results, we propose that NaF reduces sperm motility, capacitation, and the acrosome reaction leading to poor fertilization and suppressed embryonic development.
Databáze:	OpenAIRE
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