Cryptogein-Induced Transcriptional Reprogramming in Tobacco Is Light Dependent
| Autor: | Stijn Morsa, Christian Triantaphylidès, Frank Van Breusegem, Brigitte Ksas, Catherine Stassen, Michaël Vandorpe, Frank A. Hoeberichts, Céline Davoine |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Hypersensitive response
Antioxidant Photoinhibition Physiology Nicotiana tabacum medicine.medical_treatment Plant Science Fungal Proteins chemistry.chemical_compound Gene Expression Regulation Plant Tobacco Signaling and Response Genetics medicine Oxylipins Chlorophyll fluorescence Disease Resistance Glutathione Transferase Plant Diseases biology food and beverages Glycosyltransferases Glutathione biology.organism_classification Biosynthetic Pathways Elicitor Cell biology Biochemistry chemistry Systemic acquired resistance |
| Zdroj: | Plant Physiology. 163:263-275 |
| ISSN: | 1532-2548 |
| DOI: | 10.1104/pp.113.217240 |
| Popis: | The fungal elicitor cryptogein triggers a light-dependent hypersensitive response in tobacco (Nicotiana tabacum). To assess the effect of light on this nonhost resistance in more detail, we studied various aspects of the response under dark and light conditions using the tobacco-cryptogein experimental system. Here, we show that light drastically alters the plant’s transcriptional response to cryptogein, notably by dampening the induction of genes involved in multiple processes, such as ethylene biosynthesis, secondary metabolism, and glutathione turnover. Furthermore, chlorophyll fluorescence measurements demonstrated that quantum yield and functioning of the light-harvesting antennae decreased simultaneously, indicating that photoinhibition underlies the observed decreased photosynthesis and that photooxidative damage might be involved in the establishment of the altered response. Analysis of the isomer distribution of hydroxy fatty acids illustrated that, in the light, lipid peroxidation was predominantly due to the production of singlet oxygen. Differences in (reduced) glutathione concentrations and the rapid development of symptoms in the light when cryptogein was coinfiltrated with glutathione biosynthesis inhibitors suggest that glutathione might become a limiting factor during the cryptogein-induced hypersensitive response in the dark and that this response might be modified by an increased antioxidant availability in the light. |
| Databáze: | OpenAIRE |
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