Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury
| Autor: | Stefanie Trella, Camelia-Maria Monoranu, Michael Bohnert, Simone Bohnert, Werner Schmitz, Christoph Wirth, Benjamin Ondruschka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Neurofilament Traumatic brain injury Immunocytochemistry Autopsy CSF Enzyme-Linked Immunosorbent Assay Biofluid Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Neurofilament Proteins Brain Injuries Traumatic medicine Humans ddc:610 030216 legal & forensic medicine Aged Aged 80 and over biology Forensic neuropathology business.industry Myelin Basic Protein Biomarker Middle Aged medicine.disease Immunohistochemistry Myelin basic protein Staining Forensic neurotraumatology Case-Control Studies biology.protein Original Article Female business 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | International Journal of Legal Medicine |
| ISSN: | 1437-1596 0937-9827 |
| Popis: | The aim of this study was to investigate if the biomarkers myelin basic protein (MBP) and neurofilament-H (NF-H) yielded informative value in forensic diagnostics when examining cadaveric cerebrospinal fluid (CSF) biochemically via an enzyme-linked immunosorbent assay (ELISA) and comparing the corresponding brain tissue in fatal traumatic brain injury (TBI) autopsy cases by immunocytochemistry versus immunohistochemistry. In 21 trauma and 19 control cases, CSF was collected semi-sterile after suboccipital puncture and brain specimens after preparation. The CSF MBP (p = 0.006) and NF-H (p = 0.0002) levels after TBI were significantly higher than those in cardiovascular controls. Immunohistochemical staining against MBP and against NF-H was performed on cortical and subcortical samples from also biochemically investigated cases (5 TBI cases/5 controls). Compared to the controls, the TBI cases showed a visually reduced staining reaction against MBP or repeatedly ruptured neurofilaments against NF-H. Immunocytochemical tests showed MBP-positive phagocytizing macrophages in CSF with a survival time of > 24 h. In addition, numerous TMEM119-positive microglia could be detected with different degrees of staining intensity in the CSF of trauma cases. As a result, we were able to document that elevated levels of MBP and NF-H in the CSF should be considered as usefulneuroinjurybiomarkers of traumatic brain injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink