Optimization of an air-liquid interface in vitro cell co-culture model to estimate the hazard of aerosol exposures
| Autor: | He, Rui-Wen, Braakhuis, Hedwig M, Vandebriel, Rob J, Staal, Yvonne C M, Gremmer, Eric R, Fokkens, Paul H B, Kemp, Claudia, Vermeulen, Jolanda, Westerink, Remco H S, Cassee, Flemming R, Sub Biomolecular Imaging, IRAS OH Toxicology, dIRAS RA-1 |
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| Přispěvatelé: | Sub Biomolecular Imaging, IRAS OH Toxicology, dIRAS RA-1 |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Atmospheric Science
Environmental Engineering 010504 meteorology & atmospheric sciences Lipopolysaccharide Air–liquid interface Epithelial cells 010501 environmental sciences 01 natural sciences Barrier function chemistry.chemical_compound medicine Environmental Chemistry General Materials Science Viability assay 0105 earth and related environmental sciences Inhalation exposure Fluid Flow and Transfer Processes Lung Tight junction Chemistry Macrophages Mechanical Engineering respiratory system Pollution medicine.anatomical_structure Cell culture Toxicity Biophysics Aerosol exposures Co-culture |
| Zdroj: | Journal of Aerosol Science, 153, 1. Elsevier Limited Journal of Aerosol Science |
| ISSN: | 0021-8502 |
| Popis: | Inhalation exposure to environmental and occupational aerosol contaminants is associated with many respiratory health problems. To realistically mimic long-term inhalation exposure for toxicity testing, lung epithelial cells need to maintained and exposed under air-liquid interface (ALI) conditions for a prolonged period of time. In addition, to study cellular responses to aerosol particles, lung epithelial cells have to be co-cultured with macrophages. To that aim, we evaluated human bronchial epithelial Calu-3, 16HBE14o- (16HBE), H292, and BEAS-2B cell lines with respect to epithelial morphology, barrier function and cell viability under prolonged ALI culture conditions. Only the Calu-3 cells can retain the monolayer structure and maintain a strong tight junction under long-term ALI culture at least up to 2 weeks. As such, Calu-3 cells were applied as the structural barrier to create co-culture models with human monocyte-derived macrophages (MDMs) and THP-1 derived macrophages (TDMs). Adhesion of macrophages onto the epithelial monolayer was allowed for 4 h with a density of 5 × 104 macrophages/cm2. In comparison to the Calu-3 mono-culture model, Calu-3 + TDM and Calu-3 + MDM co-culture models showed an increased sensitivity in inflammatory responses to lipopolysaccharide (LPS) aerosol at Day 1 of co-culture, with the Calu-3 + MDM model giving a stronger response than Calu-3 + TDM. Therefore, the epithelial monolayer integrity and increased sensitivity make the Calu-3 + MDM co-culture model a preferred option for ALI exposure to inhaled aerosols for toxicity testing. |
| Databáze: | OpenAIRE |
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