Infection-induced membrane ruffling initiates danger and immune signaling via the mechanosensor PIEZO1
| Autor: | Lalitha Tadala, Dorothee Langenbach, Mirjam Dannborg, Ramón Cervantes-Rivera, Atin Sharma, Kevin Vieth, Lisa M. Rieckmann, Alkwin Wanders, David A. Cisneros, Andrea Puhar |
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| Rok vydání: | 2021 |
| Předmět: |
Listeria
Cell- och molekylärbiologi Adenosine Triphosphate/metabolism Ion Channels/metabolism Mechanotransduction Cellular General Biochemistry Genetics and Molecular Biology Ion Channels Microbiology in the medical area Adenosine Triphosphate danger signals Mikrobiologi inom det medicinska området Mechanotransduction Cellular/physiology Cell Membrane/metabolism Cell Membrane Immunology in the medical area Cell biology [CP] Biological Transport plasma membrane ruffles Immunologi inom det medicinska området Immunology [CP] intestinal epithelial cells mechanosensing PIEZO1 extracellular ATP invasive pathogens Shigella immune detection Cell and Molecular Biology Signal Transduction |
| Zdroj: | Tadala, L, Langenbach, D, Dannborg, M, Cervantes-Rivera, R, Sharma, A, Vieth, K, Rieckmann, L M, Wanders, A, Cisneros, D A & Puhar, A 2022, ' Infection-induced membrane ruffling initiates danger and immune signaling via the mechanosensor PIEZO1 ', Cell reports, vol. 40, no. 6, 111173 . https://doi.org/10.1016/j.celrep.2022.111173 |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2022.111173 |
| Popis: | Microorganisms are generally sensed by receptors recognizing microbial molecules, which evoke changes in cellular activities and gene expression. Bacterial pathogens induce secretion of the danger signal ATP as an early alert response of intestinal epithelial cells, initiating overt inflammation. However, what triggers ATP secretion during infection is unclear. Here we show that the inherently mechanosensitive plasma membrane channel PIEZO1 acts as a sensor for bacterial entry. PIEZO1 is mechanically activated by invasion-induced membrane ruffles upstream of Ca2+ influx and ATP secretion. Mimicking mechanical stimuli of pathogen uptake with sterile beads equally elicits ATP secretion. Chemical or genetic PIEZO1 inactivation inhibits mechanically induced ATP secretion. Moreover, chemical or mechanical PIEZO1 activation evokes gene expression in immune and barrier pathways. Thus, mechanosensation of invasion-induced plasma membrane distortion initiates immune signaling upon infection, independently of detection of microbial molecules. Hence, PIEZO1-dependent detection of infection is driven by physical signals instead of chemical ligands. |
| Databáze: | OpenAIRE |
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