A High-Dimensional Atlas of Human T Cell Diversity Reveals Tissue-Specific Trafficking and Cytokine Signatures
| Autor: | Tony Kiat Hon Lim, Eng Huat Tan, David E. Ong, Daniela Cerny, Frances Sheau Huei Lim, Florent Ginhoux, Katja Fink, Sriram Narayanan, Ern Yu Tan, Evan W. Newell, Wen Qi Ho, Michael Thomas Wong, Angela Takano, Bien Keem Tan, Maria A. Curotto de Lafaille, Karen Wei Weng Teng, Henry K.K. Tan, Jerry Kok Yen Chan, Peng Chung Cheow, John Kit Chung Tam, Rosslyn Anicete, Chung Yip Chan, Ser Yee Lee, Antonio Bertoletti, Naomi McGovern |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lymphoid Tissue Cellular differentiation medicine.medical_treatment T cell Immunology Receptors Lymphocyte Homing Biology Lymphocyte Activation Mass Spectrometry Mice 03 medical and health sciences 0302 clinical medicine Immune system Cell Movement T-Lymphocyte Subsets medicine Animals Humans Immunology and Allergy Cell Lineage Mass cytometry Receptor Cells Cultured Cell Differentiation Biodiversity T-Lymphocytes Helper-Inducer T helper cell Phenotype Cell biology Blood 030104 developmental biology Infectious Diseases medicine.anatomical_structure Cytokine Organ Specificity Cytokines Transcriptome Biomarkers 030215 immunology |
| Zdroj: | Immunity. 45:442-456 |
| ISSN: | 1074-7613 |
| Popis: | Depending on the tissue microenvironment, T cells can differentiate into highly diverse subsets expressing unique trafficking receptors and cytokines. Studies of human lymphocytes have primarily focused on a limited number of parameters in blood, representing an incomplete view of the human immune system. Here, we have utilized mass cytometry to simultaneously analyze T cell trafficking and functional markers across eight different human tissues, including blood, lymphoid, and non-lymphoid tissues. These data have revealed that combinatorial expression of trafficking receptors and cytokines better defines tissue specificity. Notably, we identified numerous T helper cell subsets with overlapping cytokine expression, but only specific cytokine combinations are secreted regardless of tissue type. This indicates that T cell lineages defined in mouse models cannot be clearly distinguished in humans. Overall, our data uncover a plethora of tissue immune signatures and provide a systemic map of how T cell phenotypes are altered throughout the human body. |
| Databáze: | OpenAIRE |
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