Med19 promotes gastric cancer progression and cellular growth
| Autor: | Xiang-fu Ding, Xue-dong Fang, Guo-min Huang, Ying Shi, Jun-an Li |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Mediator Complex
Cell growth Cancer General Medicine Biology medicine.disease Molecular biology Immunohistochemistry Small hairpin RNA Downregulation and upregulation RNA interference Cell culture Cyclin-dependent kinase Stomach Neoplasms Cell Line Tumor Gene Knockdown Techniques Cancer cell Genetics medicine biology.protein Disease Progression Humans |
| Zdroj: | Gene. 504(2) |
| ISSN: | 1879-0038 |
| Popis: | Med19 was a member of the Mediator complex which forms the bridge between transcriptional activators and RNA polymerase II. We aim to investigate the functional role of Med19 in the progression of human gastric carcinoma. The correlation between Med19 expression and clinicopathologic features in 60 gastric carcinoma specimens was analyzed by using immunohistochemistry. Recombinant lentivirus expressing Med19 short hairpin RNA (shRNA) was constructed and infected into human gastric carcinoma SGC7901 and MGC803 cells. MTT, colony formation and cell cycle analysis were used to study the effect of Med19 shRNA on gastric cancer cell proliferation. Expression of Med19 was associated with tumor size, cancer cell differentiation, and TNM stages (P |
| Databáze: | OpenAIRE |
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