A B220+ CD117+ CD19- hematopoietic progenitor with potent lymphoid and myeloid developmental potential
| Autor: | Gina Balciunaite, Steffen Massa, Antonius G. Rolink, Rod Ceredig |
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| Rok vydání: | 2005 |
| Předmět: |
Stromal cell
Myeloid Lymphoid Tissue T-Lymphocytes Immunology Antigens CD19 Biology Interleukin 21 Mice Mice Congenic medicine Immunology and Allergy Cytotoxic T cell Animals Antigens Ly Lectins C-Type Myeloid Cells Lymphopoiesis Antigens B cell Interleukin 3 B-Lymphocytes Mice Inbred BALB C Proteins Cell Differentiation Hematopoietic Stem Cells Molecular biology Mice Inbred C57BL Proto-Oncogene Proteins c-kit medicine.anatomical_structure Antigens Surface Leukocyte Common Antigens Female Bone marrow NK Cell Lectin-Like Receptor Subfamily B |
| Zdroj: | European journal of immunology. 35(7) |
| ISSN: | 0014-2980 |
| Popis: | In this report, we identify in the bone marrow (BM) of normal mice a subpopulation of B220+ CD117+ CD19- NK1.1- cells with potent lymphoid and myeloid developmental potential. These cells represent 0.1-0.2% of nucleated BM cells. By limiting dilution analysis in the presence of the appropriate combination of stromal cells and cytokines, 1 in 5-10 sorted cells formed B cells, 1 in 10-15 formed T cells and 1 in 5-10 generated macrophages. When cultured on a mixture of OP9 stroma and OP9 stromal cells expressing the Notch ligand Delta-like-1, single cells generated both T and B cells. Following intravenous infusion, freshly sorted cells transiently reconstituted both the T and B cell progenitor compartments, generating cohorts of mature T and B lymphocytes. The relationship between B220+ CD117+ CD19- NK1.1- cells of wild-type mice and other multi-lineage BM progenitors is discussed. |
| Databáze: | OpenAIRE |
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