Integrative Analysis of Fecal Metagenomics and Metabolomics in Colorectal Cancer
| Autor: | Juan M. Falcón-Pérez, Luis Bujanda, Anais Crespo, Cristina Alonso, Joaquín Cubiella, Mauro D'Amato, Marc Clos-Garcia, Koldo Garcia, Marta Iruarrizaga-Lejarreta, Águeda Iglesias |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty Colorectal cancer microbiome integration Gut flora Diagnostic tools lcsh:RC254-282 Article 03 medical and health sciences 0302 clinical medicine Metabolomics Internal medicine medicine Microbiome Feces biology business.industry Advanced adenomas Early disease Lachnospiraceae biology.organism_classification Omics lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease metabolomics digestive system diseases omics 030104 developmental biology omics integration Metagenomics 030220 oncology & carcinogenesis business multiomics |
| Zdroj: | Cancers, Vol 12, Iss 1142, p 1142 (2020) Cancers Volume 12 Issue 5 |
| ISSN: | 1556-5068 |
| DOI: | 10.2139/ssrn.3520024 |
| Popis: | Although colorectal cancer (CRC) is the second leading cause of death in developed countries, current diagnostic tests for early disease stages are suboptimal. We have performed a combination of UHPLC-MS metabolomics and 16S microbiome analyses on 224 feces samples in order to identify early biomarkers for both advanced adenomas (AD) and CRC. We report differences in fecal levels of cholesteryl esters and sphingolipids in CRC. We identified Fusobacterium, Parvimonas and Staphylococcus to be increased in CRC patients and Lachnospiraceae family to be reduced. We finally described Adlercreutzia to be more abundant in AD patients&rsquo feces. Integration of metabolomics and microbiome data revealed tight interactions between bacteria and host and performed better than FOB test for CRC diagnosis. This study identifies potential early biomarkers that outperform current diagnostic tools and frame them into the stablished gut microbiota role in CRC pathogenesis. |
| Databáze: | OpenAIRE |
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