Modulation of antibiotic resistance by the essential oil of Ocimum gratissimum L. in association with light-emitting diodes (LED) lights
| Autor: | Priscilla Ramos Freitas, Marcela Christofoli, Lívia Maria Garcia Leandro, Rizelle de Oliveira Barros, Jaime Ribeiro-Filho, Henrique Douglas Melo Coutinho, Janaína Esmeraldo Rocha, Camila Fonseca Bezerra, Paulo Pereira, Maria Karollyna do Nascimento Silva Leandro, Viviane Bomfim Bezerra, Marcello Iriti, José J. S. Aguiar, Jessica Horana F Feitosa, Edinardo F.F. Matias |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
medicine.drug_class Antibiotics Microbial Sensitivity Tests medicine.disease_cause 01 natural sciences Gas Chromatography-Mass Spectrometry General Biochemistry Genetics and Molecular Biology law.invention 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Anti-Infective Agents law Drug Resistance Bacterial Escherichia coli Oils Volatile medicine Humans Food science Essential oil biology Ocimum gratissimum Drug Synergism biology.organism_classification Anti-Bacterial Agents 0104 chemical sciences Eugenol 010404 medicinal & biomolecular chemistry Ocimum chemistry Phytochemical Antibacterial activity |
| Zdroj: | Zeitschrift für Naturforschung C. 75:377-387 |
| ISSN: | 1865-7125 0939-5075 |
| Popis: | This study aimed to evaluate the antibacterial and antibiotic-enhancing effects of the essential oil obtained from Ocimum gratissimum L. (OEOg) alone or in association with light-emitting diodes (LED) lights. The essential oil was obtained by hydrodistillation and its chemical composition analysed by gas chromatography coupled to mass spectrometry. The antibacterial and antibiotic-enhancing activities against multiresistant strains of Staphylococcus aureus and Escherichia coli were evaluated by the gaseous contact method. The analysis of the photoinductive effect on the antibacterial activity of the OEOg and antibiotics was assessed through exposure to different LED lights (red, blue and yellow). The phytochemical analysis identified five compounds, including eugenol, as the major constituent. The OEOg caused a significant inhibition of the halo, indicating a direct antibacterial effect. Exposure to the LED lights significantly enhanced the activity of the OEOg against E. coli. On the other hand, the action of the essential oil against S. aureus was enhanced by exposure to both blue and yellow lights. The effects of LED light exposure on the activity of conventional antibiotics varied significantly according to the drug and the bacterial strain. However, most combinations of LED lights and the OEOg presented synergistic effects against resistant bacterial strains, indicating enhanced antibacterial activity. Thus, these in vitro findings suggest that both OEOg and LED lights have promising antibacterial effects. Nevertheless, further research is required to evaluate in vivo the potential of these therapies for the treatment of infectious skin diseases. |
| Databáze: | OpenAIRE |
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