The effects of Levovist and DD-723 in activating platelets and damaging hepatic cells of rats
| Autor: | Shigeo Ookawara, Kouichirou Shigeta, Nobuyuki Taniguchi, Kouichi Itoh, Kiyoka Omoto |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Iron Contrast Media In Vitro Techniques Ferric Compounds Polysaccharides medicine Animals Radiology Nuclear Medicine and imaging Platelet Platelet activation Rats Wistar Whole blood Ultrasonography Microbubbles Radiological and Ultrasound Technology business.industry Ultrasound Kupffer cell Oxides Femoral Vein Flow Cytometry Platelet Activation Rats Microscopy Electron P-Selectin medicine.anatomical_structure Liver Hepatocyte Hepatic stellate cell Hepatocytes business |
| Zdroj: | Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine. 24(7) |
| ISSN: | 0278-4297 |
| Popis: | OBJECTIVE The purpose of this study was to compare platelet activation and hepatic cell damage produced by 2 ultrasonographic contrast agents with flow cytometric and ultrastructural analysis. METHODS Suspension samples were made by mixing Levovist (SH U508A; Schering AG, Berlin, Germany) or DD-723 (Nycomed; Amersham Health, Princeton, NJ) with whole blood. The final concentrations of Levovist in citrated whole blood were 0, 15, and 75 mg/mL, and those of DD-723 were 0, 5, and 50 microL/mL. After exposure to ultrasound in vitro, flow cytometric analysis was performed to determine the concentration of the CD62P activation-specific antigen. To compare the hepatic cell damage associated with these 2 agents, we divided 15 rats into 5 groups as follows: group 1, sham operation; group 2, Levovist injection only; group 3, DD-723 injection only; group 4, Levovist injection (contrast agent) and ultrasound exposure; and group 5, DD-723 injection and ultrasound exposure. The ultrasonographic contrast agents Levovist and DD-723 were administered through the femoral vein and sonicated continuously for the first minute; this was followed by sweeping for 5 minutes 10 seconds after the contrast agent was injected. The rats were perfused via the heart with a fixative solution immediately after the sweeping, and then the liver was excised; the specimens were studied with electron and light microscopy. RESULTS The percentage of CD62P-expressing platelets increased in both contrast agent-ultrasound exposure groups, and the percentage of CD62P-expressing platelets was greater in the Levovist group. We observed vacuolation and round deposits in the hepatocytes in both contrast agent-ultrasound exposure groups. Microbubbles were observed in the rat Kupffer cells, and a few hepatocytes were seen unexpectedly in the DD-723 group but were found in neither the Kupffer cells nor the hepatocytes in the Levovist group. CONCLUSIONS Both contrast agents, Levovist and DD-723, produced platelet activation and structural change in the rat hepatic cells, but only the microbubbles of DD-723 were taken up by the Kupffer cells and a few hepatocytes. |
| Databáze: | OpenAIRE |
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