Ethanol Extract ofAlismatis rhizomeInhibits Adipocyte Differentiation of OP9 Cells

Autor: Yeon-Ju Park, Young-Rae Lee, Jeong-Mi Kim, Hyuncheol Oh, Kang-Beom Kwon, Ha-Rim Kim, Dong-Sung Lee, Hye-Jung Kim, Do-Gon Ryu, Mi-Seong Kim, Youn-Chul Kim, Sei-Hoon Yang, Jin-Ki Hwang
Rok vydání: 2014
Předmět:
Zdroj: Evidence-based Complementary and Alternative Medicine : eCAM
Evidence-Based Complementary and Alternative Medicine, Vol 2014 (2014)
ISSN: 1741-4288
1741-427X
Popis: The rhizome ofAlisma orientale (Alismatis rhizome)has been used in Asia for promoting diuresis to eliminate dampness from the lower-jiaoand to expel heat. In this study, an ethanol extract of the rhizome ofAlisma orientale(AOE) was prepared and its effects on adipocyte differentiation of OP9 cells were investigated. Treatment with AOE in a differentiation medium for 5 days resulted in dose-dependent inhibition of lipid droplet formation in OP9 cells. Furthermore, AOE significantly inhibited adipocyte differentiation by downregulating the expression of the master transcription factor of adipogenesis, peroxisome proliferation-activity receptorγ(PPARγ), and related genes, including CCAAT/enhancer binding proteinβ(C/EBPβ), fatty acid-binding protein (aP2), and fatty acid synthase (FAS). AOE exerted its inhibitory effects primarily during the early adipogenesis stage (days 1-2), at which time it also exerted dose-dependent inhibition of the expression of C/EBPβ, a protein related to the inhibition of mitotic clonal expansion. Additionally, AOE decreased the expression of autophagy-related proteins, including beclin 1, and the autophagy-related genes, (Atg)7andAtg12. Our results indicate that AOE’s inhibitory effects on adipocyte differentiation of OP9 cells are mediated by reduced C/EBPβexpression, causing inhibition of mitotic clonal expansion and autophagy.
Databáze: OpenAIRE
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