CD4+ CD25+ Treg regulate the contribution of CD8+ T-cell subsets in repopulation of the lymphopenic environment
| Autor: | Antonio Lanzavecchia, Federica Sallusto, Afonso R. M. Almeida, Ilja F. Ciernik |
|---|---|
| Přispěvatelé: | Repositório da Universidade de Lisboa |
| Rok vydání: | 2010 |
| Předmět: |
Adoptive cell transfer
Cell signaling CD3 Complex Cell Survival Immunology Cell Communication CD8-Positive T-Lymphocytes Biology T-Lymphocytes Regulatory CD4+ Mice 03 medical and health sciences 0302 clinical medicine Immune system Lymphopenia Animals Regeneration Immunology and Allergy Cytotoxic T cell IL-2 receptor 030304 developmental biology Mice Knockout 0303 health sciences CD25+ T-cells Interleukin-2 Receptor alpha Subunit CD8+ Adoptive Transfer 3. Good health Cell biology Mice Inbred C57BL Treg Cd4 cd25 CD4 Antigens Repopulation Immunologic Memory CD8 030215 immunology |
| Zdroj: | Eur J Immunol Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | ©2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Peripheral T-cell expansion is of major relevance for immune function after lymphopenia. In order to promote regeneration, the process should result in a peripheral T-cell pool with a similar subpopulation structure as before lymphopenia. We investigated the repopulation of the CD8+ central-memory T cells (TCM) and effector-memory T cells (TEM) pools after adoptive transfer of sorted CD8+ T cells from naïve, TCM and TEM subsets into T-cell-deficient hosts. We show that the initial kinetics of expansion are distinct for each subset and that the contribution to the repopulation of the CD81+ T-cell pool by the progeny of each subset is not a mere function of its initial expansion. We demonstrate that CD4+ CD25+ Treg play a major role in the repopulation of the CD8+ T-cell pool and that CD8+ T-cell subsets impact on each other. In the absence of CD4+ CD25+ Treg, a small fraction of naïve CD81 T cells strongly proliferates, correlating with further expansion and differentiation of co-expanding CD8+ T cells. CD4+ CD25+ Treg suppress these responses and lead to controlled repopulation, contributing decisively to the maintenance of recovered TCM and TEM fractions, and leading to repopulation of each pool with progeny of its own kind. This work was in part supported by the Swiss National Science Foundation (Grants no. 31-126027 to A. L. and 31-116440 to F. S.) and the Cancer League of Zurich, Switzerland to I. F. C. The Institute for Research in Biomedicine is supported by the Helmut Horten Foundation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|