Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse
| Autor: | T. L. Murphy, Natasha Libina, Gregory J. Anderson, Jane Gitschier, Yien-Ming Kuo, L. Cowley, Candice C. Askwith, Chris D. Vulpe |
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| Rok vydání: | 1999 |
| Předmět: |
Hephaestin
Anemia Iron Molecular Sequence Data Mutant Mice Duodenal cytochrome B Genetics medicine Animals Humans Amino Acid Sequence Cloning Molecular Intestinal Mucosa Aceruloplasminemia Sequence Homology Amino Acid biology fungi Ceruloplasmin Chromosome Mapping Membrane Proteins Biological Transport DMT1 medicine.disease Intestinal epithelium Mice Mutant Strains Cell biology Mice Inbred C57BL Biochemistry biology.protein |
| Zdroj: | Nature Genetics. 21:195-199 |
| ISSN: | 1546-1718 1061-4036 |
| DOI: | 10.1038/5979 |
| Popis: | Iron is essential for many cellular functions; consequently, disturbances of iron homeostasis, leading to either iron deficiency or iron overload, can have significant clinical consequences. Despite the clinical prevalence of these disorders, the mechanism by which dietary iron is absorbed into the body is poorly understood. We have identified a key component in intestinal iron transport by study of the sex-linked anaemia (sla) mouse, which has a block in intestinal iron transport. Mice carrying the sla mutation develop moderate to severe microcytic hypochromic anaemia. Although these mice take up iron from the intestinal lumen into mature epithelial cells normally, the subsequent exit of iron into the circulation is diminished. As a result, iron accumulates in enterocytes and is lost during turnover of the intestinal epithelium. Biochemical studies have failed to identify the underlying difference between sla and normal mice, therefore, we used a genetic approach to identify the gene mutant in sla mice. We describe here a novel gene, Heph, encoding a transmembrane-bound ceruloplasmin homologue that is mutant in the sla mouse and highly expressed in intestine. We suggest that the hephaestin protein is a multicopper ferroxidase necessary for iron egress from intestinal enterocytes into the circulation and that it is an important link between copper and iron metabolism in mammals. |
| Databáze: | OpenAIRE |
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