Cloning and characterization of a FAD-monooxygenase gene ( cadA ) involved in degradation of chloranilic acid (2,5-dichloro-3,6-dihydroxybenzo-1,4-quinone) in Pseudomonas putida TQ07
| Autor: | Rodríguez-Uribe B, Gloria Soberón-Chávez, Galán-Wong Lj, Treviño-Quintanilla Lg |
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| Rok vydání: | 2002 |
| Předmět: |
Transposable element
Molecular Sequence Data Mutant Applied Microbiology and Biotechnology chemistry.chemical_compound Benzoquinones Amino Acid Sequence Cloning Molecular biology Pseudomonas putida Pseudomonas Sequence Analysis DNA General Medicine biology.organism_classification Mutagenesis Insertional Biodegradation Environmental Biochemistry chemistry Chloranilic acid Pseudomonadales Chromosomal region DNA Transposable Elements Oxygenases Biotechnology Pseudomonadaceae |
| Zdroj: | Applied Microbiology and Biotechnology. 59:545-550 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-002-1040-6 |
| Popis: | A bacterium culture was isolated on the basis of its ability to degrade chloranilic acid, and was later identified as Pseudomonas putida (TQ07). Several transposon insertion mutants unable to degrade chloranilic acid were selected. The characterization of the site of insertion of one of these mutants led to the identification of the cadA gene encoding an enzyme with significant homology with FAD-monooxygenases involved in the degradation of aromatic and chloroaromatic compounds. The finding that, after replacing the mutant allele with the wild-type one, the strain recovered the wild-type pattern of "halo" formation (a zone of clearing color on agar plates around TQ07 colonies that degrade chloranilic acid) and degradation of chloranilic acid, unequivocally assigned cadA a function in the metabolism of this compound. We also found that most of the transposon insertion mutants unable to degrade chloranilic acid are clustered in a 10-kb region of the P. putida genome that is encoded in a megaplasmid or in an unstable chromosomal region. |
| Databáze: | OpenAIRE |
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