Quantitative Analysis of Somatostatin and Dopamine Receptors Gene Expression Levels in Non-functioning Pituitary Tumors and Association with Clinical and Molecular Aggressiveness Features
| Autor: | Eva Venegas-Moreno, Miguel A. Japón, Raúl M. Luque, Justo P. Castaño, David A. Cano, Pablo Remón-Ruiz, Noelia Gros-Herguido, Florinda Roldan, Alfonso Soto-Moreno, Alvaro Flores-Martínez, Ainara Madrazo-Atutxa, Eugenio Cárdenas-Valdepeñas, Ariel Kaen, M Carmen Vazquez-Borrego, Elena Dios |
|---|---|
| Přispěvatelé: | Universidad de Sevilla. Departamento de Medicina, [Flores-Martinez,Á, Venegas-Moreno,E, Dios,E, Remón-Ruiz,P, Gros-Herguido,N, Madrazo-Atutxa,A, Cano,DA, Soto-Moreno,A] Unidad de Gestión de Endocrinología y Nutrición, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain. [Vázquez-Borrego,MC, Castaño,JP, Luque,RM] Maimonides Institute for Biomedical Research of Córdoba (IMIBIC), Córdoba, Spain. [Vázquez-Borrego,MC, Luque,RM] Department of Cell Biology, Physiology and Immunology, University of Córdoba, Córdoba, Spain. [Vázquez-Borrego,MC, Luque,RM] Hospital Universitario Reina Sofía, Córdoba, Spain. [Vázquez-Borrego,MC, Luque,RM] CIBER Fisiopatología de la Obesidad y Nutrición (CIBERObn), Córdoba, Spain. [Japón,MA] Department of Pathology, Hospital Universitario Virgen del Rocío, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain. [Kaen,A, Cárdenas-Valdepeñas,E] Servicio de Neurocirugía, Hospital Universitario Virgen del Rocío, Sevilla, Spain. [Roldán,F] Servicio de Radiología, Hospital Universitario Virgen del Rocío, Sevilla, Spain., This work was supported by grants from the ISCIII-Subdirección General de Evaluación y Fomento de la Investigación co-funded with Fondos FEDER (PI16/00175 to A.S.-M. and D.A.C, PI16/00264 to R.M.L.), the Sistema Andaluz de Salud (A-0006-2017 and A-0055-2018 to A.S.-M, C-0015-2014 and RC-0006-2018 to D.A.C.), Ministerio de Ciencia, Innovación (BFU2016-80360-R to J.P.C., PID2019-105564RB-I00 to R.M.L.) and Junta de Andalucía (BIO-0139 to J.P.C. and R.M.L.), and CIBERobn. CIBER is an initiative of Instituto de Salud Carlos III, Ministerio de Sanidad, Servicios Sociales e Igualdad, Spain. Part of the analyses included in this study was carried out within the REMAH (‘Spanish molecular registry of pituitary adenomas’) project, supported by Novartis Oncology as well as by the Andalusian and Spanish Societies of Endocrinology and Nutrition (SAEDYN and SEEN, respectively). |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Medicine
Agonistas de dopamina Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunohistochemistry [Medical Subject Headings] Somatostatin receptor Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] 0302 clinical medicine fluids and secretions Invasion Gene expression dopamine receptor Receptor General Medicine invasion somatostatin receptor Somatostatin Receptores de somatostatina Dopamine agonists Dopamine receptor Immunohistochemistry hormones hormone substitutes and hormone antagonists medicine.drug medicine.medical_specialty endocrine system Receptores dopaminérgicos 030209 endocrinology & metabolism pituitary tumor Article Non-functioning pituitary tumors 03 medical and health sciences Dopamine Internal medicine parasitic diseases medicine Neoplasias hipofisarias Geographical Locations::Geographic Locations::Europe::Spain [Medical Subject Headings] Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Neurotransmitter Agents::Dopamine Agents::Dopamine Agonists [Medical Subject Headings] business.industry Pituitary tumors fungi lcsh:R Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction [Medical Subject Headings] Andalucía medicine.disease Endocrinology Diseases::Neoplasms::Neoplasms by Site::Endocrine Gland Neoplasms::Pituitary Neoplasms [Medical Subject Headings] non-functioning pituitary tumors business 030217 neurology & neurosurgery Pituitary tumor |
| Zdroj: | Journal of Clinical Medicine, Vol 9, Iss 3052, p 3052 (2020) Journal of Clinical Medicine Volume 9 Issue 9 idUS. Depósito de Investigación de la Universidad de Sevilla instname idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) |
| ISSN: | 2077-0383 |
| Popis: | The primary treatment for non-functioning pituitary tumors (NFPTs) is surgery, but it is often unsuccessful. Previous studies have reported that NFPTs express receptors for somatostatin (SST1-5) and dopamine (DRDs) providing a rationale for the use of dopamine agonists and somatostatin analogues. Here, we systematically assessed SST1-5 and DRDs expression by real-time quantitative PCR (RT-qPCR) in a large group of patients with NFPTs (n = 113) and analyzed their potential association with clinical and molecular aggressiveness features. SST1-5 expression was also evaluated by immunohistochemistry. SST3 was the predominant SST subtype detected, followed by SST2, SST5, and SST1. DRD2 was the dominant DRD subtype, followed by DRD4, DRD5, and DRD1. A substantial proportion of NFPTs displayed marked expression of SST2 and SST5. No major association between SSTs and DRDs expression and clinical and molecular aggressiveness features was observed in NFPTs ISCIII-Subdirección General de Evaluación y Fomento de la Investigación co-funded with Fondos FEDER PI16/00175 to A.S.-M. and D.A.C; PI16/00264 to R.M.L. Sistema Andaluz de Salud A-0006-2017 and A-0055-2018 to A.S.-M, C-0015-2014 and RC-0006-2018 to D.A.C. Ministerio de Ciencia, Innovación BFU2016-80360-R to J.P.C.; PID2019-105564RB-I00 to R.M.L. Junta de Andalucía BIO-0139 to J.P.C. and R.M.L. Instituto de Salud Carlos III, Ministerio de Sanidad, Servicios Sociales e Igualdad, Spain |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|