Laminin expression by two clones isolated from the colon carcinoma cell line lovo that differ in metastatic potential and basement-membrane organization
| Autor: | Marie-France Jacquier, Jean-François Doré, Lionel Remy, Jean-Claude Lissitzky, Daemi N, Pierre-Marie Martin, Maryse Bailly, C. Bignon |
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| Rok vydání: | 1992 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty medicine.drug_class Mice Nude Basement membrane organization Biology Monoclonal antibody Basement Membrane Immunocompromised Host Mice Laminin Tumor Cells Cultured medicine Animals Humans Neoplasm Metastasis Basement membrane Molecular biology Rats medicine.anatomical_structure Animals Newborn Oncology Cell culture Colonic Neoplasms biology.protein Immunohistochemistry Antibody Clone (B-cell biology) Cell Division |
| Zdroj: | International Journal of Cancer. 51:204-212 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910510207 |
| Popis: | In the present report we describe the characteristics of 2 clones, E2 and C5, isolated from the human colon adenocarcinoma cell line LoVo. When grafted to immunosuppressed newborn rats, these clones formed tumors that varied with regard to differentiation rate, basement-membrane organization and lung metastatic potential. Production and distribution of laminin by E2, C5 and related tumors was studied by immunohistochemistry with an anti-laminin monoclonal antibody 4C12 (MAb 4C12). In lowly metastatic E2-derived tumors, strong regular stainings were observed which were strictly peri-tumoral and corresponded to the basal lamina. Since the antibody interacted with human laminin (the graft) but not with rat laminin (the host), this result indicated that basement-membrane laminin was supplied mainly by tumor-cell synthesis. In highly metastatic C5-derived tumors, the staining obtained with MAb 4C12 was peri-cellular and unorganized. Laminin synthesis by E2 and C5 cells in sub-cultures or soon after dissociation from explanted tumors was studied by metabolic labelling with 35S-methionine under steady-state conditions followed by immunoprecipitation and SDS-PAGE. High-molecular-weight laminin comprised by disulfide-linked A and B chains, i.e., heterotrimeric laminin, was found in cell lysates and in the secretion medium of cell lines and tumor cells. In addition, B1B2 dimers and free B chains were observed in cell lysates. Quantitatively, laminin expression by E2 and C5 clones or tumor cells was not significantly different. These findings suggest that basement-membrane defects in invasive clone LoVo C5 were not due to laminin under-expression. |
| Databáze: | OpenAIRE |
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