| Autor: |
Deblina Biswas, Jeongmin Heo, Pilgyu Sang, Prasanta Dey, Kayoung Han, Jong Hwan Ko, Sang Min Won, Donghee Son, Minah Suh, Hyung Sik Kim, Jong G. Ok, Hui Joon Park, Hyoung Won Baac |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
ACS sensors. 7(10) |
| ISSN: |
2379-3694 |
| Popis: |
Abnormal formation of solid thrombus inside a blood vessel can cause thrombotic morbidity and mortality. This necessitates early stage diagnosis, which requires quantitative assessment with a small volume, for effective therapy with low risk to unwanted development of various diseases. We propose a micro-ultrasonic diagnosis using an all-optical ultrasound-based spectral sensing (AOUSS) technique for sensitive and quantitative characterization of early stage and whole blood coagulation. The AOUSS technique detects and analyzes minute viscoelastic variations of blood at a micro-ultrasonic spot (100 μm) defined by laser-generated focused ultrasound (LGFU). This utilizes (1) a uniquely designed optical transducer configuration for frequency-spectral matching and wideband operation (6 dB widths: 7-32 MHz and d.c. ∼ 46 MHz, respectively) and (2) an empirical mode decomposition (EMD)-based signal process particularly adapted to nonstationary LGFU signals backscattered from the spot. An EMD-derived spectral analysis enables one to assess viscoelastic variations during the initiation of fibrin formation, which occurs at a very early stage of blood coagulation (1 min) with high sensitivity (frequency transition per storage modulus increment = 8.81 MHz/MPa). Our results exhibit strong agreement with those obtained by conventional rheometry (Pearson's |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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