Alternations in oxidative stress, apoptosis, and innate-immune gene expression at mRNA levels in subadult tiger puffer (Takifugu rubripes) under two different rearing systems
| Autor: | Changtao Guan, Zhai Jieming, Bin Huang, Yudong Jia, Qiqi Jing |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Takifugu rubripes Gene Expression Zoology Apoptosis Aquaculture Aquatic Science medicine.disease_cause 03 medical and health sciences Gene expression medicine Animals Environmental Chemistry RNA Messenger Innate immune system biology Tiger business.industry Gene Expression Profiling Recirculating aquaculture system 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate Takifugu Hsp70 Oxidative Stress 030104 developmental biology 040102 fisheries 0401 agriculture forestry and fisheries Female business Oxidative stress |
| Zdroj: | Fish & Shellfish Immunology. 92:756-764 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2019.07.016 |
| Popis: | Tiger puffer (Takifugu rubripes) is one of the major aquaculture fish species in China due to its high economic value. In this study, the transcriptions of hepatic antioxidant enzyme, stress, apoptosis, and immune-related genes of sub-adult tiger puffers (Takifugu rubripes) were evaluated under two different rearing systems [offshore sea cage aquaculture system (OSCS) and recirculating aquaculture system (RAS)]. Results showed that the mRNA expression levels of the antioxidant enzyme (mn-sod, cu/zn-sod, gpx, and gr) and stress-related (hsp70 and hsp90) genes of male tiger puffers reared in the OSCS were significantly higher than female fish reared in the OSCS and fish reared in the RAS. The anti-apoptotic gene bcl2 exhibited the similar results. By contrast, the mRNAs of the pro-apoptotic genes (p53, caspase8, caspase9, and caspase3) of male tiger puffers reared in the OSCS were significantly lower than female fish reared in the OSCS and fish reared in the RAS. Male tiger puffers reared in the OSCS displayed significantly higher complement components (c3) and inflammatory cytokine (il-6) mRNAs, whereas B-cell activating factor (baf) and tumor necrosis factor α (tnf-α) mRNAs remained unchanged. Meanwhile, the mRNA levels of pro-apoptotic (bax, caspase8) and immunity-related (c3, il-6 and il-7) genes of female tiger puffers reared in the OSCS were significantly lower and higher than female fish reared in the RAS, respectively. In conclusion, the hepatic antioxidant, anti-apoptosis, and innate immunity of tiger puffers reared in the OSCS were better than fish in the RAS, male tiger puffer obtained the best values. These results expand the knowledge on the combined RAS and OSCS alternative aquaculture model for tiger puffers and aid in their management in captive. |
| Databáze: | OpenAIRE |
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