Localization of cAMP-dependent protein kinase in the actin and microtubule cytoskeletons in mouse hippocampal neurons
Autor: | Sakura Saburi, Shigeaki Kato, Mutsumi Takano, Akihiro Harada, Reiko Sato-Harada, Takashi Sato |
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Rok vydání: | 2002 |
Předmět: |
A-kinase-anchoring protein
Cytochalasin B Protein subunit Growth Cones macromolecular substances Biology Hippocampus Microtubules Mice chemistry.chemical_compound Microtubule-associated protein 2 Animals Tissue Distribution Cytoskeleton Protein kinase A Cells Cultured Neurons General Neuroscience Colocalization Dendrites Cyclic AMP-Dependent Protein Kinases Actins Cell biology chemistry Cytoplasm Subcellular Fractions |
Zdroj: | Neuroscience Letters. 325:83-86 |
ISSN: | 0304-3940 |
DOI: | 10.1016/s0304-3940(02)00248-3 |
Popis: | Cyclic adenosine monophosphate-dependent protein kinase (PKA) is involved in various biological functions in neurons. To investigate the subcellular localization of PKA, we stained cultured hippocampal neurons with anti-PKA catalytic subunit antisera. PKA catalytic subunit colocalized with microtubules (MTs) in dendrites as well as with the actin filaments (F-actin) in growth cones. After treatment with cytochalasin B, the colocalization of PKA catalytic subunits with MTs was enhanced, whereas the colocalization with F-actin was suppressed. This result indicates that PKA is anchored to the actin and MT cytoskeletons, and disruption of F-actin releases PKA to the cytoplasm, which then leads to an increase in the amount of PKA in MT domains in the neuron. |
Databáze: | OpenAIRE |
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