Dopaminergic ventral tegmental neurons modulated by methylphenidate
| Autor: | M. T. Benitez, Nachum Dafny, C. Reyes Vazquez, B. Prieto-Gomez, Pamela Yang, A. M. Vazquez-Alvarez |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Patch-Clamp Techniques Dopamine Kainate receptor In Vitro Techniques Motor Activity General Biochemistry Genetics and Molecular Biology GABA Antagonists Rats Sprague-Dawley chemistry.chemical_compound medicine Animals Humans Premovement neuronal activity Rats Wistar General Pharmacology Toxicology and Pharmaceutics Child Amphetamine Neurons Afferent Pathways Dose-Response Relationship Drug Ventral Tegmental Area Dopaminergic General Medicine Rats Ventral tegmental area Electrophysiology medicine.anatomical_structure nervous system chemistry Methylphenidate Saclofen Central Nervous System Stimulants Excitatory Amino Acid Antagonists Neuroscience medicine.drug Picrotoxin |
| Zdroj: | Life Sciences. 74:1581-1592 |
| ISSN: | 0024-3205 |
| Popis: | Treatment of psychostimulants leads to the development of behavioral sensitization, an augmented behavioral response to drug re-administration. The induction of behavioral sensitization to psychostimulants such as amphetamine and cocaine occurs at the ventral tegmental area's dopaminergic neurons (VTA-DA). Currently, there is limited experimental data about the physiological properties of methylphenidate (MPD) on VTA-DA neurons. Behavioral and electrophysiological experiments using male rats were performed before and after MPD treatment. The behavioral experiment included dose-response (0.6, 2.5, and 10.0 mg/kg MPD) study to select the most effective dose for the electrophysiological study. Methylphenidate increased locomotion in typical dose response characteristics. Based on this experiment, the 10.0 mg/kg MPD was used in two types of electrophysiological recordings: 1) intracellular recording of neuronal activity performed on horizontal 275–300 μm brain slices and 2) whole-cell patch clamping before and after electrical stimulation to study post-synaptic currents on neurophysiologically identified VTA-DA neurons. Methylphenidate suppressed the neuronal activity of these neurons for 210±30 sec. Stimulation of the prefrontal cortex afferent fibers to these VTA-DA neurons in the presence of TTX, saclofen, and picrotoxin led to the conclusion that this input is mediated via NMDA and kainate/AMPA receptors and may participate to induce behavioral sensitization to psychostimulants. |
| Databáze: | OpenAIRE |
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